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3.A.7.10.1
The E. coli plasmid R64 IncI conjugation system

Accession Number:Q7DJM1
Protein Name:TraL protein
Length:115
Molecular Weight:12364.00
Species:Plasmid R64 of E. coli [2492]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate protein polypeptide chain

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References (6)

[1] “Requirement of a limited segment of the sog gene for plasmid R64 conjugation.”  Narahara K.et.al.   9281491
[2] “Determination of the nick site at oriT of IncI1 plasmid R64: global similarity of oriT structures of IncI1 and IncP plasmids.”  Furuya N.et.al.   1917882
[3] “Mutational analysis of the R64 oriT region: requirement for precise location of the NikA-binding sequence.”  Furuya N.et.al.   9393692
[4] “Purification and characterization of thin pili of IncI1 plasmids ColIb-P9 and R64: formation of PilV-specific cell aggregates by type IV pili.”  Yoshida T.et.al.   9603870
[5] “Concerted action of three distinct domains in the DNA cleaving-joining reaction catalyzed by relaxase (TraI) of conjugative plasmid RP4.”  Pansegrau W.et.al.   8300611
[6] “Complete nucleotide sequence of Birmingham IncP alpha plasmids. Compilation and comparative analysis.”  Pansegrau W.et.al.   8014987

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNMHKSLVAV LGLILISSTA SAATCRAGSA AQAGSNTGYE RARAAADAWS QRENDVSSSL 
61:	QSCLSRIKKI SINLPQFPSL DDILSQLENQ VCDAVVDKVN EKLPGNIDPW KDYNL