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3.A.7.10.1
The E. coli plasmid R64 IncI conjugation system

Accession Number:Q7DJM3
Protein Name:TraJ aka Nucleotide-binding protein
Length:382
Molecular Weight:42539.00
Species:Plasmid R64 of E. coli [2492]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate protein polypeptide chain

Cross database links:

Pfam: PF00437   

Gene Ontology

GO:0005622 C:intracellular
GO:0005524 F:ATP binding
GO:0006810 P:transport

References (5)

[1] “Determination of the nick site at oriT of IncI1 plasmid R64: global similarity of oriT structures of IncI1 and IncP plasmids.”  Furuya N.et.al.   1917882
[2] “Mutational analysis of the R64 oriT region: requirement for precise location of the NikA-binding sequence.”  Furuya N.et.al.   9393692
[3] “Purification and characterization of thin pili of IncI1 plasmids ColIb-P9 and R64: formation of PilV-specific cell aggregates by type IV pili.”  Yoshida T.et.al.   9603870
[4] “Concerted action of three distinct domains in the DNA cleaving-joining reaction catalyzed by relaxase (TraI) of conjugative plasmid RP4.”  Pansegrau W.et.al.   8300611
[5] “Complete nucleotide sequence of Birmingham IncP alpha plasmids. Compilation and comparative analysis.”  Pansegrau W.et.al.   8014987

External Searches:

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNIPDEFGLF PFQRFTADEL RHFFVWCAAH KVSDVDLTGG SPVSVSRFGR RVRCSSATLP 
61:	TTLMSSLIDE LFGREVIPRV LAGNPVDRTI QINGDASGRY GLKRGERIRL RSHLIQGTSG 
121:	AEEKAISITM RVIPTEIPDI LSMNIEPDLL EAMVCKSGLG FVCGETGSGK STLCSALYRY 
181:	IMDNFPDAKI VTYEDPVEYI LGNENDLLPP HQAEIGRDVV SFAAGLRSAV RRNPEIIGVG 
241:	EIRDNETADA AVQAGNTGHY CLSTMHTKSP GETLARLLGL FPPVIRDSMA WAVLSLLQFI 
301:	LVQVLVRTND GGRKAVREYI VINDELRDNL SGMPHAEWGH HIDAIIRQEK RRIRDQILEM 
361:	YIRNEVDRRE AILFIPPGEL RS