5.A.2.1.2 The DsbI (223 aas and 4 TMSs in a 3 + 1 arrangement)/DsbL (222 aas and 1 N-terminal TMS) disulfide bond oxidoreductase/thio:disulfide interchange protein together form a dithiol oxidase system, acting on the periplasmic enzyme arylsulfate sulfotransferase, AssT (598 aas, 1 N-terminal TMS, Q8FDI4) which requires a disulfide bond for activity. The 3-d structure of DsbL at 1.55 Å resolution has been determined. DsbL is one of the most reactive oxidizing thioredoxin-like protein known (Grimshaw et al. 2008). These three enzymes are encoded within a tricistronic operon.
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Accession Number: | Q8FDI3 |
Protein Name: | Protein-disulfide oxidoreductase DsbI |
Length: | 223 |
Molecular Weight: | 25117.00 |
Species: | Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) [199310] |
Number of TMSs: | 4 |
Location1 / Topology2 / Orientation3: |
Cell inner membrane1 / Multi-pass membrane protein2 |
Substrate |
electron |
---|
1: MGIKGMWKDL RTSPVDTLVR WQEQRLLWLL MAVAMGALII LAHSFFQIYL YMAPCEQCVY
61: IRYAMFVMVI GGLVAAINPK NIILKLIGCV MAFYGSILGL KFSLKLNDIH HAVHNPDPDS
121: LFGVQGCSTD PTFPFNLPLA QWAPNWFKPT GDCGYDAPIV PDGVTLSSTQ QWFVEMYQQS
181: EGWYLLPPWH FMNMAQACML AFGMCLVLLV IMSGAWALKI IRG