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2.A.78.1.2
Branched chain amino acid efflux pump, BrnEF (extrudes L-methionine, L-isoleucine, L-leucine and L-valine) (Trötschel et al., 2005).  Its deletion results in a slight increase (13%) in lysine production and a decrease in cell mass (Xiao et al. 2020).

Accession Number:Q8NTP8
Protein Name:BrnF
Length:251
Molecular Weight:27333.00
Species:Corynebacterium glutamicum (Brevibacterium flavum) [1718]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Apical cell membrane1 / Multi-pass membrane protein2
Substrate L-methionine, L-isoleucine, valine, leucine

Cross database links:

RefSeq: NP_599511.1    YP_224558.1   
Entrez Gene ID: 1021322    3342679   
Pfam: PF03591   
BioCyc: CGLU196627:CG0314-MONOMER   
KEGG: cgb:cg0314    cgl:NCgl0254   

References (2)

[1] “The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.”  Kalinowski J.et.al.   12948626
[2] “Export of L-isoleucine from Corynebacterium glutamicum: a two-gene-encoded member of a new translocator family.”  Kennerknecht N.et.al.   12081967

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MQKTQEIHSS LEVSPSKAAL EPDDKGYRRY EIAQGLKTSL AAGLGMYPIG IAFGLLVIQY 
61:	GYEWWAAPLF SGLIFAGSTE MLVIALVVGA APLGAIALTT LLVNFRHVFY AFSFPLHVVK 
121:	NPIARFYSVF ALIDEAYAVT AARPAGWSAW RLISMQIAFH SYWVFGGLTG VAIAELIPFE 
181:	IKGLEFALCS LFVTLTLDSC RTKKQIPSLL LAGLSFTIAL VVIPGQALFA ALLIFLGLLT 
241:	IRYFFLGKAA K