4.D.3.1.4
Trimeric cellulose synthase coomplex for primary cell wall synthesis, CESA1, 3 and 6 (Watanabe et al. 2015). CESA1 is of 1084 aas and 8 TMSs while other CESA subunits are of similar size. All three are catalytic
subunit of the cellulose synthase complex. Required
for beta-1,4-glucan microfibril crystallization, a major mechanism of primary cell wall formation. Required during embryogenesis for cell elongation, orientation of cell
expansion and complex cell wall formation (Bashline et al. 2014).
determined the structure of a poplar cellulose synthase CesA homotrimer
that suggests a molecular basis for cellulose microfibril formation.
This complex, stabilized by cytosolic plant-conserved regions and
helical exchange within the transmembrane segments, forms three channels
occupied by nascent cellulose polymers. Secretion steers the polymers
toward a common exit point, which could facilitate protofibril
formation. CesA's N-terminal domains assemble into a cytosolic stalk
that interacts with a microtubule-tethering protein and may thus be
involved in CesA localization.
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| Accession Number: | Q941L0 |
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| Protein Name: | Cellulose synthase A catalytic subunit 3 [UDP-forming] |
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| Length: | 1065 |
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| Molecular Weight: | 119683.00 |
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| Species: | Arabidopsis thaliana (Mouse-ear cress) [3702] |
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| Number of TMSs: | 8 |
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| Location1 / Topology2 / Orientation3: |
Cell membrane1 / Multi-pass membrane protein2 |
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| Substrate |
(1->4)-beta-D-glucan |
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1: MESEGETAGK PMKNIVPQTC QICSDNVGKT VDGDRFVACD ICSFPVCRPC YEYERKDGNQ
61: SCPQCKTRYK RLKGSPAIPG DKDEDGLADE GTVEFNYPQK EKISERMLGW HLTRGKGEEM
121: GEPQYDKEVS HNHLPRLTSR QDTSGEFSAA SPERLSVSST IAGGKRLPYS SDVNQSPNRR
181: IVDPVGLGNV AWKERVDGWK MKQEKNTGPV STQAASERGG VDIDASTDIL ADEALLNDEA
241: RQPLSRKVSI PSSRINPYRM VIMLRLVILC LFLHYRITNP VPNAFALWLV SVICEIWFAL
301: SWILDQFPKW FPVNRETYLD RLALRYDREG EPSQLAAVDI FVSTVDPLKE PPLVTANTVL
361: SILAVDYPVD KVSCYVSDDG AAMLSFESLA ETSEFARKWV PFCKKYSIEP RAPEWYFAAK
421: IDYLKDKVQT SFVKDRRAMK REYEEFKIRI NALVSKALKC PEEGWVMQDG TPWPGNNTRD
481: HPGMIQVFLG QNGGLDAEGN ELPRLVYVSR EKRPGFQHHK KAGAMNALVR VSAVLTNGPF
541: ILNLDCDHYI NNSKALREAM CFLMDPNLGK QVCYVQFPQR FDGIDKNDRY ANRNTVFFDI
601: NLRGLDGIQG PVYVGTGCVF NRTALYGYEP PIKVKHKKPS LLSKLCGGSR KKNSKAKKES
661: DKKKSGRHTD STVPVFNLDD IEEGVEGAGF DDEKALLMSQ MSLEKRFGQS AVFVASTLME
721: NGGVPPSATP ENLLKEAIHV ISCGYEDKSD WGMEIGWIYG SVTEDILTGF KMHARGWRSI
781: YCMPKLPAFK GSAPINLSDR LNQVLRWALG SVEILFSRHC PIWYGYNGRL KFLERFAYVN
841: TTIYPITSIP LLMYCTLPAV CLFTNQFIIP QISNIASIWF LSLFLSIFAT GILEMRWSGV
901: GIDEWWRNEQ FWVIGGVSAH LFAVFQGILK VLAGIDTNFT VTSKASDEDG DFAELYLFKW
961: TTLLIPPTTL LIVNLVGVVA GVSYAINSGY QSWGPLFGKL FFAFWVIVHL YPFLKGLMGR
1021: QNRTPTIVVV WSVLLASIFS LLWVRIDPFT SRVTGPDILE CGINC