4.D.3.1.4
Trimeric cellulose synthase coomplex for primary cell wall synthesis, CESA1, 3 and 6 (Watanabe et al. 2015). CESA1 is of 1084 aas and 8 TMSs while other CESA subunits are of similar size. All three are catalytic
subunit of the cellulose synthase complex. Required
for beta-1,4-glucan microfibril crystallization, a major mechanism of primary cell wall formation. Required during embryogenesis for cell elongation, orientation of cell
expansion and complex cell wall formation (Bashline et al. 2014).
determined the structure of a poplar cellulose synthase CesA homotrimer
that suggests a molecular basis for cellulose microfibril formation.
This complex, stabilized by cytosolic plant-conserved regions and
helical exchange within the transmembrane segments, forms three channels
occupied by nascent cellulose polymers. Secretion steers the polymers
toward a common exit point, which could facilitate protofibril
formation. CesA's N-terminal domains assemble into a cytosolic stalk
that interacts with a microtubule-tethering protein and may thus be
involved in CesA localization.
|
| Accession Number: | Q94JQ6 |
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| Protein Name: | Cellulose synthase A catalytic subunit 6 [UDP-forming] |
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| Length: | 1084 |
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| Molecular Weight: | 122502.00 |
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| Species: | Arabidopsis thaliana (Mouse-ear cress) [3702] |
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| Number of TMSs: | 8 |
|---|
| Location1 / Topology2 / Orientation3: |
Cell membrane1 / Multi-pass membrane protein2 |
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| Substrate |
(1->4)-beta-D-glucan |
|---|
1: MNTGGRLIAG SHNRNEFVLI NADENARIRS VQELSGQTCQ ICRDEIELTV DGEPFVACNE
61: CAFPVCRPCY EYERREGNQA CPQCKTRFKR LKGSPRVEGD EEEDDIDDLD NEFEYGNNGI
121: GFDQVSEGMS ISRRNSGFPQ SDLDSAPPGS QIPLLTYGDE DVEISSDRHA LIVPPSLGGH
181: GNRVHPVSLS DPTVAAHPRP MVPQKDLAVY GYGSVAWKDR MEEWKRKQNE KLQVVRHEGD
241: PDFEDGDDAD FPMMDEGRQP LSRKIPIKSS KINPYRMLIV LRLVILGLFF HYRILHPVKD
301: AYALWLISVI CEIWFAVSWV LDQFPKWYPI ERETYLDRLS LRYEKEGKPS GLSPVDVFVS
361: TVDPLKEPPL ITANTVLSIL AVDYPVDKVA CYVSDDGAAM LTFEALSETA EFARKWVPFC
421: KKYCIEPRAP EWYFCHKMDY LKNKVHPAFV RERRAMKRDY EEFKVKINAL VATAQKVPED
481: GWTMQDGTPW PGNSVRDHPG MIQVFLGSDG VRDVENNELP RLVYVSREKR PGFDHHKKAG
541: AMNSLIRVSG VLSNAPYLLN VDCDHYINNS KALREAMCFM MDPQSGKKIC YVQFPQRFDG
601: IDRHDRYSNR NVVFFDINMK GLDGLQGPIY VGTGCVFRRQ ALYGFDAPKK KKGPRKTCNC
661: WPKWCLLCFG SRKNRKAKTV AADKKKKNRE ASKQIHALEN IEEGRVTKGS NVEQSTEAMQ
721: MKLEKKFGQS PVFVASARME NGGMARNASP ACLLKEAIQV ISCGYEDKTE WGKEIGWIYG
781: SVTEDILTGF KMHSHGWRSV YCTPKLAAFK GSAPINLSDR LHQVLRWALG SVEIFLSRHC
841: PIWYGYGGGL KWLERLSYIN SVVYPWTSLP LIVYCSLPAI CLLTGKFIVP EISNYASILF
901: MALFSSIAIT GILEMQWGKV GIDDWWRNEQ FWVIGGVSAH LFALFQGLLK VLAGVDTNFT
961: VTSKAADDGE FSDLYLFKWT SLLIPPMTLL IINVIGVIVG VSDAISNGYD SWGPLFGRLF
1021: FALWVIIHLY PFLKGLLGKQ DRMPTIIVVW SILLASILTL LWVRVNPFVA KGGPILEICG
1081: LDCL