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3.A.1.1.10
Alginate (MW 27,000 Da) (and Alginate oligosaccharides) uptake porter. Sphingomonas species A1 is a 'pit-forming' bacterium that directly incorporates alginate into its cytoplasm through a pit-dependent transport system, termed a 'superchannel' (Murata et al., 2008). The pit is a novel organ acquired through the fluidity and reconstitution of cell surface molecules, and through cooperation with the transport machinery in the cells. It confers upon bacterial cells a more efficient way to secure and assimilate macromolecules (Murata et al., 2008).  The substrate-transport characteristics and quaternary structure of AlgM1M2SS with AlgQ1 have been determined (Maruyama et al. 2015). The addition of poly- or oligoalginate enhanced the ATPase activity of reconstituted AlgM1M2SS coupled with one of the periplasmic solute-binding proteins, AlgQ1 or AlgQ2. External fluorescence-labeled oligoalginates were specifically imported into AlgM1M2SS-containing proteoliposomes in the presence of AlgQ2, ATP, and Mg2+. The crystal structure of AlgQ2-bound AlgM1M2SS adopts an inward-facing conformation. The interaction between AlgQ2 and AlgM1M2SS induces the formation of an alginate-binding tunnel-like structure accessible to solvent. The translocation route inside the transmembrane domains contains charged residues suitable for the import of acidic saccharides (Maruyama et al. 2015).

Accession Number:Q9KWT6
Protein Name:AlgQ1
Length:526
Molecular Weight:60285.00
Species:Sphingomonas sp [28214]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Alginate

Cross database links:

Pfam: PF01547   

Gene Ontology

GO:0005215 F:transporter activity
GO:0006810 P:transport

References (2)

[1] “A novel bacterial ATP-binding cassette transporter system that allows uptake of macromolecules.”  Momma K.et.al.   10869078
[2] “Direct evidence for Sphingomonas sp. A1 periplasmic proteins as macromolecule-binding proteins associated with the ABC transporter: molecular insights into alginate transport in the periplasm.”  Momma K.et.al.   15794643
Structure:
1Y3N   1Y3P   1Y3Q   3A09   3VLU   3VLV   3VLW     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKKLVLAMAI GSSALMFAAP SVVAREATWV TEKPLTLKIH MHFRDKWVWD ENWPVAREVA 
61:	RLTNVKLVGV ANRAATNSQE QFNLMMASGQ LPDIVGGDNL KDKFIRYGME GAFIPLNKLI 
121:	DQNAPNLKAF FKTHPEVQRA ITAPDGNIYY LPYVPDGLVS RGYFIRQDWL DKLHLKTPQT 
181:	VDELYTVLKA FKEKDPNGNG KADEIPFINR DPEEVFRLVN FWGARSTGSN TWMDFYVENG 
241:	KIKHPFAEVA FKDGIKHVAQ WYKEGLIDPE IFTRKARSRE QTFGNNIGGM THDWFASTAL 
301:	FNDALSKNIP GFKLVPMAPP INSKGQRWEE DARQIPRPDG WAITATNKNP VETIKLFDFY 
361:	FGPKGRELSN FGVPGLTYDI KNGKPVYKDT VLKAAQPVNN QMYDIGAQIP IGFWQDYEYE 
421:	RQWTNDVALQ GIDMYIKNKY VLPQFTGVNL TVEEREIYDK YWPDVKTYMF EMGQSWVMGT 
481:	KDPEKTWNDY QQQLKNRGFY QVMIVMQKAY DRQYGGAAKP AQVGAK