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3.A.1.1.10
Alginate (MW 27,000 Da) (and Alginate oligosaccharides) uptake porter. Sphingomonas species A1 is a 'pit-forming' bacterium that directly incorporates alginate into its cytoplasm through a pit-dependent transport system, termed a 'superchannel' (Murata et al., 2008). The pit is a novel organ acquired through the fluidity and reconstitution of cell surface molecules, and through cooperation with the transport machinery in the cells. It confers upon bacterial cells a more efficient way to secure and assimilate macromolecules (Murata et al., 2008).  The substrate-transport characteristics and quaternary structure of AlgM1M2SS with AlgQ1 have been determined (Maruyama et al. 2015). The addition of poly- or oligoalginate enhanced the ATPase activity of reconstituted AlgM1M2SS coupled with one of the periplasmic solute-binding proteins, AlgQ1 or AlgQ2. External fluorescence-labeled oligoalginates were specifically imported into AlgM1M2SS-containing proteoliposomes in the presence of AlgQ2, ATP, and Mg2+. The crystal structure of AlgQ2-bound AlgM1M2SS adopts an inward-facing conformation. The interaction between AlgQ2 and AlgM1M2SS induces the formation of an alginate-binding tunnel-like structure accessible to solvent. The translocation route inside the transmembrane domains contains charged residues suitable for the import of acidic saccharides (Maruyama et al. 2015).

Accession Number:Q9KWT9
Protein Name:AlgS
Length:363
Molecular Weight:39524.00
Species:Sphingomonas sp [28214]
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Peripheral membrane protein2
Substrate Alginate

Cross database links:

Pfam: PF00005    PF03459   

Gene Ontology

GO:0043190 C:ATP-binding cassette (ABC) transporter complex
GO:0005524 F:ATP binding
GO:0016887 F:ATPase activity
GO:0016820 F:hydrolase activity, acting on acid anhydrid...
GO:0006810 P:transport

References (1)

[1] “A novel bacterial ATP-binding cassette transporter system that allows uptake of macromolecules.”  Momma K.et.al.   10869078
Structure:
4TQU   4TQV   4XIG   4XTC     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MVASVSIQNV VKRYDKTTVV HGVSLDIEPG EFVVLVGPSG CGKSTTLRMV AGLEEISGGT 
61:	IRIDGRVIND LAPKDRDVAM VFQNYALYPH LNVRDNISFG LRLKRTKKSV IDAAVKTAAD 
121:	ILGLQPLLER KPSDLSGGQR QRVAMGRAIV RDPKVFLFDE PLSNLDAKLR TQMRAEIKRL 
181:	HQRLGTTVIY VTHDQVEAMT LADRIVVMRD GLIEQIGKPM DLFLHPANTF VASFIGSPPM 
241:	NLMPARIAVD STQHVELNGG NRISLLPRAG THLAPGQEVV FGIRPEDVTL DGVEGSERAQ 
301:	IKATVDIVEP LGSESILHAT VGDHSLVVKV GGLNEVHPGD PVTLHVDLTR VHLFDAQSQA 
361:	SIY