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3.A.1.12.5
Uptake system for glycine-betaine (high affinity) and proline (low affinity) (OpuAA-OpuABC) or BusAA-ABC of Lactococcus lactis). BusAA, the ATPase subunit, has a C-terminal tandem cystathionine β-synthase (CBS) domain which is the cytoplasmic K+ sensor for osmotic stress (osmotic strength)while the BusABC subunit has the membrane and receptor domains fused to each other (Biemans-Oldehinkel et al., 2006; Mahmood et al., 2006; Gul et al. 2012). An N-terminal amphipathic α-helix of OpuA is necessary for high activity but is not critical for biogenesis or the ionic regulation of transport (Gul et al., 2012). ATP and glycine betaine dependences of conformational changes have been examined (Tassis et al. 2020).

Accession Number:Q9RQ05
Protein Name:BusAB aka OPUABC
Length:573
Molecular Weight:62128.00
Species:Lactococcus lactis [1358]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Proline, Glycine betaine

Cross database links:

RefSeq: YP_001032363.1   
Entrez Gene ID: 4797696   
Pfam: PF00528    PF04069   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005488 F:binding
GO:0005215 F:transporter activity
GO:0006810 P:transport

References (2)

[1] “Genetic and biochemical characterization of a high-affinity betaine uptake system (BusA) in Lactococcus lactis reveals a new functional organization within bacterial ABC transporters.”  Obis D.et.al.   10515910
[2] “Characterization of OpuA, a glycine-betaine uptake system of Lactococcus lactis.”  Bouvier J.et.al.   10939245

External Searches:

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MIDLVIGKIP LANWVSSATD WITSTFSSGF DVIQKSGTVL MNGITGALTA VPFWLMIAVV 
61:	TILAILVSGK KFAFPLFAFI GLCLIANQGL WSDLMSTITL VLLSSLLSII IGVPLGIWMA 
121:	KSELVAKIVQ PILDFMQTMP GFVYLIPAVA FFGIGVVPGV FASVIFALPP TVRMTNLGIR 
181:	QVSTELVEAA DSFGSTARQK LFKLEFPLAK GTIMAGVNQT IMLALSMVVI ASMIGAPGLG 
241:	RGVLAAVQSA DIGKGFVSGI SLVILAIIID RFTQKLNVSP LEKQGNPKLK KWKRWIAIVS 
301:	LLALIVGAFS GMSFGKKSSD KKVDLVYMNW DSEVASINVL TQAMEEHSFD VTTTALDNAV 
361:	AWQTVANSQA DGMVSAWLPN THKTQWKKYG KSVELLGPNL KGAKVGFVVP SYMNVNSIED 
421:	LTNQANKTIT GIEPGAGVMA ASENTLKSYS NLKDWKLVPS SSGAMTVALG EAIKQHKDIV 
481:	ITGWSPHWIF NKYDLKYLAD PKGTMGTSEN INTIVRKGLK KENPEAYKVL NNFNWTTKDM 
541:	ESVMLDIQNG KTPEAAAKAW IKDHQKQVDK WFK