2.A.6.2.21
The multidrug (aminoglycosides, β-lactams, fluoroquinolones, macrolides, chloramphenicol, tetracycline, erythromycin, ofloxacin, etc.) efflux pump, MexXY-OprM (Jeannot et al., 2005). The 3-d structurre of OprM (also called OprK) is known (1WP1). RND-type xenobiotic transporters recognize hydrophobic substrates such
as organic solvents by their periplasmic domains and expel them to the
external milieu (Li et al. 2006). The MexXY multidrug exporter is frequently overexpressed in ciprofloxacin resistant cells (Serra et al. 2019). Increased expression or overexpression ofMexXY-OprM efflux pumps is the leading cause of carbapenem (imigenem; meropenem) resistance (Petrova et al. 2019).
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| Accession Number: | Q9ZNG8 |
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| Protein Name: | MexY |
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| Length: | 1046 |
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| Molecular Weight: | 113117.00 |
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| Species: | Pseudomonas aeruginosa [287] |
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| Number of TMSs: | 11 |
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| Location1 / Topology2 / Orientation3: |
Cell outer membrane1 / Lipid-anchor2 |
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| Substrate |
macrolide, fluoroquinolone antibiotic, ofloxacin, ciprofloxacin, chloramphenicol, beta-lactam, imipenem, tetracycline, amino cyclitol glycoside, erythromycin A, carbapenemcarboxylic acid |
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| Pfam: |
PF00873
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[1] “Expression in Escherichia coli of a new multidrug efflux pump, MexXY, from Pseudomonas aeruginosa.” Mine T. et.al. 9925549
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1: MARFFIDRPV FAWVISLLIV LAGVLAIRFL PVAQYPDIAP PVVNVSATYP GASAKVVEEA
61: VTAIIEREMN GAPGLLYTKA TSSTGQASLT LTFRQGVNAN LAAVEVQNRL KIVESRLPES
121: VRRDGIYVEK AADSIQLIVT LTSSSGRYDA MELGEIASSN VLQALRRVEG VGKVETWGAE
181: YAMRIWPDPA KLTSMNLSAS DLVNAVRRHN ARLTVGDIGN LGVPDSAPIS ATVKVDDTLV
241: TPEQFGEIPL AHPRDGGAIR LRDVARVEFG QSEYGFVSRV NQMTATGLAV KMAPGSNAVA
301: TAKRIRATLD ELSRYFPEGV SYNIPYDTSA FVEISIRKVV STLLEAMLLV FAVMYLFMQN
361: FRATLIPTLV VPVALLGTFT VMLGLGFSIN VLTMFGMVLA IGILVDDAII VVENVERLMA
421: EEGLSPHDAT VKAMRQISGA IVGITVVLVS VFVPMAFFSG AVGNIYRQFA VTLAVSIGFS
481: AFLALSLTPA LCATLLRPID ADHHEKRGFF GWFNRAFLRL TGRYRNAVAG ILARPIRWML
541: VYTLVIGVVA LLFVRLPQAF LPEEDQGDFM IMVMQPEGTP MAETMANVGD VERYLAEHEP
601: VAYAYAVGGF SLYGDGTSSA MIFATLKDWS ERREASQHVG AIVERINQRF AGLPNRTVYA
661: MNSPPLPDLG STSGFDFRLQ DRGGVGYEAL VKARDQLLAR AAEDPRLANV MFAGQGEAPQ
721: IRLDIDRRKA ETLGVSMDEI NTTLAVMFGS DYIGDFMHGS QVRKVVVQAD RRKRLGIDDI
781: GRLHVRNEQG EMGAAGDVRQ GRLDPRPAAT DPLQRLSLVQ PRGPGRAGLQ QREAMQAMEQ
841: LMQGTARGIR PRVVRPVLRR TPVAGAQAPA LFALSVLIVF LALAALYESW SIPLAVILVV
901: PLGVLGALLG VSLRGLPNDI YFKVGLITII GLSAKNAILI IEVAKDHYQE GMSLLQATLE
961: AARLRLRPIV MTSLAFGFGV VPLALSSGAG IRAQVAIGTG VLGGIVTATV LAVFLVPLFF
1021: LVVGRLFRLR KAPRTGNSPQ IPTEQA