1.A.120.1.1
The coronaviral double membrane-spanning pore complex in the replication organelle (RO) of the viral double membrane vesicle (DMV). The pore consists of a hexameric complex. The primary constituent of each of the six subunits of the pore is the non-structural protein-3, nsp3, of 2309 aas, which may have 5 TMSs, based on a hydrophathy plot of the protein. These are clustered together about two-thrids of the way towards the C-terminus. It is the core constituent of the pore and has both its N-terminal domain of 160 kDa and a smaller C-terminal domain, which contains a ubiquitin domain (Ubi1) of 12.6 kDa, that together form the prongs of the cytoplasmic crown. nsp3 interacts with nsp4, and this complex is believed to drive membrane pairing and participate in DMV biogenesis (Wolff et al. 2020). It may associate with other viral and host proteins.
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| Accession Number: | YP_009915690.1 |
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| Protein Name: | YP_009915690.1 nsp3 [Murine hepatitis virus] |
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| Length: | 2310 |
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| Molecular Weight: | |
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| Species: | Murine hepatitis virus [11138] |
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| Number of TMSs: | 5 |
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| Substrate |
(ribonucleotide)n+m |
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1: GKKVEFNDKP KVRKIPSTRK IKITFALDAT FDSVLSKACS EFEVDKDVTL DELLDVVLDA
61: VESTLSPCKE HDVIGTKVCA LLDRLAGDYV YLFDEGGDEV IAPRMYCSFS APDDEDCVAA
121: DVVDADENQD DDAEDSAVLV ADTQEEDGVA KGQVEADSEI CVAHTGSQEE LAEPDAVGSQ
181: TPIASAEETE VGEASDREGI AEAKATVCAD AVDACPDQVE AFEIEKVEDS ILDELQTELN
241: APADKTYEDV LAFDAVCSEA LSAFYAVPSD ETHFKVCGFY SPAIERTNCW LRSTLIVMQS
301: LPLEFKDLEM QKLWLSYKAG YDQCFVDKLV KSVPKSIILP QGGYVADFAY FFLSQCSFKA
361: YANWRCLECD MELKLQGLDA MFFYGDVVSH MCKCGNSMTL LSADIPYTLH FGVRDDKFCA
421: FYTPRKVFRA ACAVDVNDCH SMAVVEGKQI DGKVVTKFIG DKFDFMVGYG MTFSMSPFEL
481: AQLYGSCITP NVCFVKGDVI KVVRLVNAEV IVNPANGRMA HGAGVAGAIA EKAGSAFIKE
541: TSDMVKAQGV CQVGECYESA GGKLCKKVLN IVGPDARGHG KQCYSLLERA YQHINKCDNV
601: VTTLISAGIF SVPTDVSLTY LLGVVTKNVI LVSNNQDDFD VIEKCQVTSV AGTKALSLQL
661: AKNLCRDVKF VTNACSSLFS ESCFVSSYDV LQEVEALRHD IQLDDDARVF VQANMDCLPT
721: DWRLVNKFDS VDGVRTIKYF ECPGGIFVSS QGKKFGYVQN GSFKEASVSQ IRALLANKVD
781: VLCTVDGVNF RSCCVAEGEV FGKTLGSVFC DGINVTKVRC SAIYKGKVFF QYSDLSEADL
841: VAVKDAFGFD EPQLLKYYTM LGMCKWSVVV CGNYFAFKQS NNNCYINVAC LMLQHLSLKF
901: PKWQWQEAWN EFRSGKPLRF VSLVLAKGSF KFNEPSDSID FMRVVLREAD LSGATCNLEF
961: VCKCGVKQEQ RKGVDAVMHF GTLDKGDLVR GYNIACTCGS KLVHCTQFNV PFLICSNTPE
1021: GRKLPDDVVA ANIFTGGSVG HYTHVKCKPK YQLYDACNVN KVSEAKGNFT DCLYLKNLKQ
1081: TFSSVLTTFY LDDVKCVEYK PDLSQYYCES GKYYTKPIIK AQFRTFEKVD GVYTNFKLVG
1141: HSIAEKLNAK LGFDCNSPFV EYKITEWPTA TGDVVLASDD LYVSRYSSGC ITFGKPVVWL
1201: GHEEASLKSL TYFNRPSVVC ENKFNVLPVD VSEPTDKGPV PAAVLVTGVP GADASAGAGI
1261: AKEQKACASA SVEDQVVTEV RQEPSVSAAD VKEVKLNGVK KPVKVEGSVV VNDPTSETKV
1321: VKSLSIVDVY DMFLTGCKYV VWTANELSRL VNSPTVREYV KWGKGKIVTP AKLLLLRDEK
1381: QEFVAPKVVK AKAIACYCAV KWFLLYCFSW IKFNTDNKVI YTTEVASKLT FKLCCLAFKN
1441: ALQTFNWSVV SRGFFLVATV FLLWFNFLYA NVILSDFYLP NIGPLPTFVG QIVAWFKTTF
1501: GVSTICDFYQ VTDLGYRSSF CNGSMVCELC FSGFDMLDNY DAINVVQHVV DRRLSFDYIS
1561: LFKLVVELVI GYSLYTVCFY PLFVLIGMQL LTTWLPEFFM LETMHWSARL FVFVANMLPA
1621: FTLLRFYIVV TAMYKVYCLC RHVMYGCSKP GCLFCYKRNR SVRVKCSTVV GGSLRYYDVM
1681: ANGGTGFCTK HQWNCLNCNS WKPGNTFITH EAAADLSKEL KRPVNPTDSA YYSVTEVKQV
1741: GCSMRLFYER DGQRVYDDVN ASLFVDMNGL LHSKVKGVPE THVVVVENEA DKAGFLGAAV
1801: FYAQSLYRPM LMVEKKLITT ANTGLSVSRT MFDLYVDSLL NVLDVDRKSL TSFVNAAHNS
1861: LKEGVQLEQV MDTFIGCARR KCAIDSDVET KSITKSVMSA VNAGVDFTDE SCNNLVPTYV
1921: KSDTIVAADL GVLIQNNAKH VQANVAKAAN VACIWSVDAF NQLSADLQHR LRKACSKTGL
1981: KIKLTYNKQE ANVPILTTPF SLKGGAVFSR MLQWLFVANL ICFIVLWALM PTYAVHKSDM
2041: QLPLYASFKV IDNGVLRDVS VTDACFANKF NQFDQWYEST FGLAYYRNSK ACPVVVAVID
2101: QDIGHTLFNV PTTVLRYGFH VLHFITHAFA TDSVQCYTPH MQIPYDNFYA SGCVLSSLCT
2161: MLAHADGTPH PYCYTGGVMH NASLYSSLAP HVRYNLASSN GYIRFPEVVS EGIVRVVRTR
2221: SMTYCRVGLC EEAEEGICFN FNRSWVLNNP YYRAMPGTFC GRNAFDLIHQ VLGGLVRPID
2281: FFALTASSVA GAILAIIVVL AFYYLIKLK