1.A.1.11.10
Plasma membrane voltage-gated, high affinity Ca2+ channel, Cch1/Mid1; activated by mating pheromones and environmental stresses; required for growth in low Ca2+ (Locke et al., 2000; Paidhungat and Garrett, 1997). Also essential for tolerance to cold stress and iron toxicity (Peiter et al., 2005). Ecm7, (448aas; 4 TMS; TC# 1.H.1.4.6), a member of the PMP-22/EMP/MP20 Claudin superfamily of transmembrane proteins that includes gamma-subunits of voltage-gated calcium channels appears to interact with Mid1 TC# 8.A.41.1.1) and regulate the activity of the Cch1/Mid1 channel (Martin et al., 2011). Ecm7p is related to members of TC families 1.H.1, 1.H.2 and 1.A.81. The two indispensable subunits, Cch1 and Mid1 are equivalent to the mammalian pore-forming α1 and auxiliary α2 /δ subunits, respectively. Cho et al. 2016 screened candidate proteins that interact with Mid1 and identified the plasma membrane H+-ATPase, Pma1 (TC#3.A.3.3.6). Mid1 co-immunoprecipitated with Pma1. At the nonpermiss, and Mid1-EGFP colocalized with Pma1-mCherry at the plasma membrane. Using a temperature-sensitive mutant, pma1-10, the membrane potential was less negative, and Ca2+ uptake was lower than in wild-type cells. Thus, Pma1 interacts physically with Cch1/Mid1 Ca2+ channels to enhance their activity via its H+-pumping activity (Cho et al. 2016).
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| Accession Number: | P50077 |
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| Protein Name: | Calcium-channel protein CCH1 |
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| Length: | 2039 |
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| Molecular Weight: | 234599.00 |
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| Species: | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [559292] |
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| Number of TMSs: | 23 |
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| Location1 / Topology2 / Orientation3: |
Cell membrane1 |
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| Substrate |
Ca2+ |
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1: MQGRKRTLTE PFEPNTNPFG DNAAVMTENV EDNSETDGNR LESKPQALVP PALNIVPPES
61: SIHSTEEKKG DEYNGNDKDS SLISNIFRTR VGRSSHENLS RPKLSLKTAS FGAAESSRRN
121: VSPSTKSAKS SSQYIDLNDE RLRRRSFSSY SRSSSRRVSN SPSSTDRPPR SAKVLSLIAA
181: DDMDDFEDLQ KGFKSAIDEE GLTWLPQLKS EKSRPVSDVG EDRGEGEQES IPDVHTPNVG
241: ASATPGSIHL TPEPAQNGSV SEGLEGSINN SRKKPSPKFF HHLSPQKEDK DQTEVIEYAE
301: DILDFETLQR KLESRPFVLY GHSLGVFSPT NPLRIKIARF LLHRRYSLLY NTLLTFYAIL
361: LAIRTYNPHN VVFLYRFSNW TDYFIFILSA CFTGNDIAKI IAFGFWDDSE MFKAYGREYK
421: SILQRSGIMK LYIYLREKYG RKLIDFIIPF RIISPGEETK YQRSSLSTSL TKPYGAKENQ
481: RPFGTPRAFA RSSWNRIDLV SSVSFWLGMF LSIKSYDTKT GIRIFKPLAI LRILRLVNVD
541: TGMPSILRGL KYGIPQLVNV SSMLVYFWIF FGILGVQIFQ GSFRRQCVWF NPEDPTDTYQ
601: YDMQFCGGYL DPVTKRKQNY IYEDGSEGSV SKGFLCPQYS KCVSNANPYN GRISFDNIVN
661: SMELVFVIMS ANTFTDLMYY TMDSDEMAAC LFFIVCIFVL TIWLLNLLIA VLVSSFEIAN
721: EEYKKKKFIY GSRKTGYVAR IVTGYWKYFK LKANQTKFPN WSQKGLAIYS HVEFIFVILI
781: ICDIGMRASV KVSTSANCNN ILLKTDRGIS IVLFIESLAR LVLYLPNMWK FLTKPSYVYD
841: FIISIITLVI SCLAVEGVLG HMYAWLSIFH ISRFYRVIIS FNLTKKLWKQ ILSNGVMIWN
901: LSSFYFFFTF LVAIIMAVYF EGVIPPEEMA DQPFGMYSLP NSFLSLFIIG STENWTDILY
961: ALQKHSPNIS STFFCSVFFI IWFLLSNSVI LNIFIALISE SMEVKEEEKR PQQIKHYLKF
1021: VYPQKIQEYT HASLVARIRK KFFGGHRNED TRDFKQFLMR GTAIMNIAQN MGELADEFKE
1081: PPSENLFKKG LSKLTIGVPS LKRLRMFANN PFYKNSDVVF TETNDINGRT YILELNEYED
1141: EKLDYLKKYP LFNYSYYFFS PQHRFRRFCQ RLVPPSTGKR TDGSRFFEDS TDLYNKRSYF
1201: HHIERDVFVF IFALATILLI VCSCYVTPLY RMHHKMGTWN WSSALDCAFI GAFSIEFIVK
1261: TVADGFIYSP NAYLRNPWNF IDFCVLISMW INLIAYLKNN GNLSRIFKGL TALRALRCLT
1321: ISNTARQTFN LVMFDGLNKI FEAGLISLSL LFPFTVWGLS IFKGRLGTCN DGSLGRADCY
1381: NEYSNSVFQW DIMSPRVYQQ PYLHLDSFAS AFSSLYQIIS LEGWVDLLEN MMNSSGIGTP
1441: ATVMGSAGNA LFLVLFNFLS MVFILNLFVS FIVNNQARTT GSAYFTIEEK AWLESQKLLS
1501: QAKPKAIPNL IELSRVRQFF YQLAVEKKNF YYASFLQVVL YLHIIMLLSR SYNPGNLIGY
1561: QGVYFMFSTS VFLIQEALHM CGEGPRLYFR QKWNSIRLSI IIIAFIMNAV AFHVPASHYW
1621: FHNIKGFFLL VIFLFIIPQN DTLTELLETA MASLPPILSL TYTWGVLFLV YAIALNQIFG
1681: LTRLGSNTTD NINFRTVIKS MIVLFRCSFG EGWNYIMADL TVSEPYCSSD DNSTYTDCGS
1741: ETYAYLLLMS WNIISMYIFV NMFVSLIIGN FSYVYRSGGS RSGINRSEIK KYIEAWSKFD
1801: TDGTGELELS YLPRIMHSFD GPLSFKIWEG RLTIKSLVEN YMEVNPDDPY DVKIDLIGLN
1861: KELNTIDKAK IIQRKLQYRR FVQSIHYTNA YNGCIRFSDL LLQIPLYTAY SARECLGIDQ
1921: YVHHLYILGK VDKYLENQRN FDVLEMVVTR WKFHCRMKRT IEPEWDVKDP TVSSHISNIN
1981: VNLEPAPGIL EREPIATPRM DYGVNNFMWS PRMNQDSTME PPEEPIDNND DSANDLIDR