1.A.1.11.29
Voltage-gated calcium channel (VDCC) of 3097 aas and 24 TMSs, Cav7 (Wheeler and Brownlee 2008). The photoreceptor potential in Chlamydomonas triggers the generation of all or no flagellar Ca2+ currents that cause membrane depolarization across the eyespot and
flagella (Sanyal et al. 2023). Modulation in membrane potential causes changes in the
flagellar waveform, and hence, alters the beating patterns of
Chlamydomonas flagella. The eyespot membrane potential is
rhodopsin-mediated and is generated by the photoreceptor Ca2+ current or P-current. However, flagellar Ca2+ currents are mediated by unidentified voltage-gated calcium (VGCC or
CaV) and potassium channels (VGKC). The voltage-dependent ion channel
that associates with ChRs to generate Ca2+ influx across the
flagella and its cellular distribution has been
identified. Sanyal et al. 2023 presented evidence on Chlamydomonas reinhardtii predicting that CrVGCC4
localizes to the eyespot and flagella and associates
with channelrhodopsins. Further in silico interactome analysis of
CrVGCCs suggested that they interact with photoreceptor
proteins, calcium signaling, and intraflagellar transport components.
Expression analysis indicated that these VGCCs and their putative
interactors can be perturbed by light stimuli. Thus, VGCCs in general, and VGCC4 in particular, might be
involved in the regulation of the phototactic response of Chlamydomonas.
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| Accession Number: | A8IHW3 |
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| Protein Name: | Voltage-gated Ca2+ channel, alpha subunit |
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| Length: | 3097 |
|---|
| Molecular Weight: | 328197.00 |
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| Species: | Chlamydomonas reinhardtii [3055] |
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| Number of TMSs: | 18 |
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| Substrate |
calcium(2+) |
|---|
1: MLLAQDPKLQ AAYDSYMASQ LMSSLTEDEM FAATSALGAV QKAYASNNGV RRPTSPSPGV
61: EAAGPSGASQ HAGAGDKDVL AAAGVTDARR KRRSSGASCS DMLGEGLGER EHSVARSGHS
121: GSARPGSSLA GRQDGEAESG RGTPLQQHPT LPSGKVPLLD SDDFGGPPLP TSAAAVAAAL
181: ALVEVKPLAD HIQRDDEHLP PLLGPPPGAP LQPAPPPGAA AAAAAATAGG VGGADVTKDN
241: KDGKDGALQQ QDSNNSLGTY DEFGDLGQYS GLRRTCIKLC SNPDFEMVVT AIIFANCVTL
301: CLYDPRQPES SGLNKKLFWA ELAFNICFSI EMLLRVVSMG SLLGYIRRPW NQFDSFMVVA
361: GYTVFVPGNS SAVNAIRALR ALRPLRTITR FESLRGVIVC FLEAIPLLAS VGGLLIFFVF
421: MYAVAAVQLF PKTYHNTCLD PEGVPLNMGE DPDMFGCGGR GHCPANYTCM LLEHQHTVNV
481: AGFDNAGLAM LSVWQALTLT GWVFMMYRTI DTYSLYVVIY YLTLVVIGAY FVLNLFLAVL
541: KIKFAKAQTL FHNQLALQRG KARRNSIMTF MSRVQTKWTE YSSKRSQASS LNSRLTSAIS
601: SKMTSVASAG SPRMSHDGKE AEGEGSKAKR HGSKDKLGSE KGHAGRRRGS ANGSAYEQRS
661: ADGFLPGSVE AEGGGDEVGG ARARVEVGSA GGYLAASKGA GSLQASREGT RDGVGSKPEW
721: TQLEPLPRPG SPVNAADRGA ARSALAGSRP SSGTPSSRQL SCSSSRVAFM PAPGEPGVRF
781: AGGDDEPGNE GDHPSARTGP RGGGGVRFGD EPASEPGGHG SDPTSPHLGA GEYTHRSSGG
841: QGSATGEIMY SPHEARTGSG LQGPAGGDGV GGAGGRTSSA DGLRPAASAE LGERTYSAAP
901: SSMGATNVAM IVMSPAEFDE FIADHPYTQR QYLRLQYRVR IFVNHNLFNQ FFMLLIFLNT
961: IFLSLEYHNQ PDSLTQALNV GNLTLTVLFT IEMVLKLFGL GFWDYIRDGF NIFDALVVTI
1021: SLLEIVLSSV GGLNAVRALR VLKALRVLRL FKLFRYMQSL RRIGEVLLSA ASSFMAIATL
1081: LILFWMVFAI VGMHVFGDKD LDTFPWPNFS TFLYSLVATW NVLNLENWQN IMYAVIRQTG
1141: YASSLFFVMW IIIGRYIFLT LFLAVTLEAF EAKYDPNAVR MGANKTGISS VLGSLRSGLS
1201: SLRSGMNSVR SSMRGSGKGR SPPDSPHGRK GGGGEGPKGG AGGAVTTAAK QPEQGKVPAG
1261: SGGKVTPATE VPSNIDDTRS DSEIGRDSEP VPPPSVLRPG IGYMADAQAA AAAAAAGNPE
1321: APRVNLKRMY TIKEWIDTGN FEVLKDGGAK DGAAAPAADG AAAAAASLSA AGAADALKAE
1381: PSTARSLKRE PSARLTGPSE PVAEGGSNKP ARGTSIIDDS AAQSLASSDA AGGNSASGEA
1441: KDVGATNGPA RPVAEGTETI RPDPTALPPS SSGGGGRSSL TGLRSNGGSP SPTWAGTGSR
1501: GRLESINRNI RRSLEGFAAE ADEEEGGDGD AAAGPRRHVL RGPSPLGGFA PSSTAAGSRN
1561: SASGMAAGSE LILTHGRTPA PLRGGGLSPD LMRMAGSFGD DSSTDDDDAK PGAGGPAGAA
1621: TAKTAAPVLA TAGSLNTPGL RSSATTTVTT ASAAEAQKLQ QQQQQQPLVT VESSEDPLGS
1681: FMDPLHLAQP HAGQPSAAGA AYGPAVAATS SIFLPSKPLP APQAYGILPG SPQPQLSLGL
1741: LSEPASGNDV VMATFGDMSS IFAQPHAEPQ QPQPQTQTQQ APPVNASRLS YELEGVDALA
1801: GWAGSAGNAH RARRSSTTVP RRHGGPAPAA AAEPQAGDRG VGSGAAPGSA EDDHLMALAG
1861: ELAAALKEQS VPQPAAPGDS DAVAAFRAQT LSAEPSAITT GGAVGWTSAS ETTSMQPTPR
1921: LKEPEAAAAA QPHADRPSAV PALHLERVAH GAPSTVPHAT AAAAAAAAAS SAFGSSGSKA
1981: SEVVPLGAAS LQDSAHSMSA SDLLGSGRRV IRAGGGAEGL LPKAPSTRLP PGEETLQNLD
2041: AYLPPGEALS RAATSNLAAR DADVKEAAAA SGLLADTLGE RIRTAAAAAR HTRGQTQEEE
2101: DELIFGLQPI RGSKDASRRS SQDDGAPRRP GSGRSGRDQK TPSPGPGGGK GDDEDGEDAG
2161: RQGDKGPPLQ RISESGSVAS RADSVNSHVS AGSRASSGGA TGTDDDGDEK SDKKRKHFRK
2221: KRRASILIEG SGKAFWIWDE DHPWRENTYW LVTHKRFEYT MFAVILANCV TLALENPYIV
2281: PGSPLDQALV WSNVGFTVIF GVEALLKSFA YTFTAYIKRV TNQVDFLIVV SSVVEIILLT
2341: ITTSVGAVSA LRVLRAFKPL RLLTRSAGMR LVFKSVTMSL MSMANVSVVC ILFFLIFAIL
2401: GVQLFSGRFY RCNDDTVAGQ TECVGNFTDP LTNSTLERHW SNDFPNFDNT GNALLCCFIT
2461: ATLNGYTEIM TNAMSAPQDV GLQPKQFINP GAFFWFFGFI VVCAFTLLNL YVGVIFSQFS
2521: KIRMLSETGS AFLTNDQNEW AELTKMVFRL KPPEKSHLPT SRLRRFCYQL VHHKYFDIFI
2581: LTIILINVGF LAATWYGEPP SYTRQKDLAN IVFMSVFAAE AVVKIFALGW MGYIKVSWNK
2641: LDFVLLVLGL LDLVVSMLQS NFLRILRVFR VQKLIALVRV ARFAQLVKSV KGIQSLFSTL
2701: VYSLPAFGNV GALIGLFFFM YAYVGTFLFG KVVGGETLNR SVNFHDFFQS LLVLTRVATG
2761: DNWTGVMFDC MVMPPDCDRA EGNCGSYLAI PYFLSFFLII SVILLNLFTA VIIETFEKTH
2821: EQEEWKLSPQ ALEDFVTLWS EYDDGSGTIQ PRHLEELLLK LDPPLGLGAY ADNKDVLRFV
2881: YDLDIPLVNG RVPFHKTAFE LVKRCSQATM PEGAIKEQID RLVDKFFHEL TAQDEMLNFS
2941: VAITVMKVQR KWRTRMRAAK LRRKNQWRKD RRDAPSYFDV STNKVSVGEK YEAYRDAQRE
3001: VAKTWEAAQV ASNKKVDKAE LARRSEPRWR FNPKYAPPSV VAATTEKTGL LGLGKRAINV
3061: NKLVGLFGLR GGLFGDGGDA DKGGRPPAAE FPAQLPD