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1.A.1.2.6
Voltage-gated K+ channel, Shaker. Shaker and Shab K+ channels are blocked by quinidine (Gomez-Lagunas, 2010). Also regulated by unsaturated fatty acids (Börjesson and Elinder, 2011).  TMSs 3 and 4 comprise the voltage sensor paddle (Xu et al. 2013).  Partially responsible for action potential repolarization during synaptic transmission (Ford and Davis 2014). Shaker K+ channels can be mutated in S4 to create an analogous "omega" pore (Held et al. 2018). The NMR structure of the isolated Shaker voltage-sensing domain in LPPG micelles has been reported (Chen et al. 2019). Substituting the first S4 arginine with a smaller amino acid opens a high-conductance pathway for solution cations in the Shaker K+ channel at rest. The cationic current does not flow through the central K+ pore and is influenced by mutation of a conserved residue in S2, suggesting that it flows through a protein pathway within the voltage-sensing domain (Tombola et al. 2005). The current can be carried by guanidinium ions, suggesting that this is the pathway for transmembrane arginine permeation. Tombola et al. 2005 proposed that when S4 moves, it ratchets between conformations in which one arginine after another occupies and occludes to ions in the narrowest part of this pathway. Specific resin acids activate and open voltage-gated channels  dependent on its exact binding dynamics (Silverå Ejneby et al. 2021).

Accession Number:P08510
Protein Name:Potassium voltage-gated channel protein Shaker
Length:655
Molecular Weight:74193.00
Species:Drosophila melanogaster (Fruit fly) [7227]
Number of TMSs:7
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate K+

Cross database links:

RefSeq: NP_001162788.1    NP_523393.3    NP_728120.1    NP_728122.1    NP_728123.1    NP_728124.1    NP_996497.1    NP_996498.1   
Entrez Gene ID: 32780   
Pfam: PF00520    PF02214   
KEGG: dme:Dmel_CG1234   

Gene Ontology

GO:0008076 C:voltage-gated potassium channel complex
GO:0005515 F:protein binding
GO:0005249 F:voltage-gated potassium channel activity
GO:0048675 P:axon extension
GO:0048150 P:behavioral response to ether
GO:0007619 P:courtship behavior
GO:0009584 P:detection of visible light
GO:0007629 P:flight behavior
GO:0008345 P:larval locomotory behavior
GO:0007611 P:learning or memory
GO:0006813 P:potassium ion transport
GO:0007637 P:proboscis extension reflex
GO:0001508 P:regulation of action potential
GO:0045187 P:regulation of circadian sleep/wake cycle, s...
GO:0060025 P:regulation of synaptic activity
GO:0050909 P:sensory perception of taste
GO:0055085 P:transmembrane transport

References (13)

[1] “Sequence of a probable potassium channel component encoded at Shaker locus of Drosophila.”  Tempel B.L.et.al.   2441471
[2] “Molecular organization of the maternal effect region of the Shaker complex of Drosophila: characterization of an I(A) channel transcript with homology to vertebrate Na(+) channel.”  Baumann A.et.al.   16453805
[3] “Shaker encodes a family of putative potassium channel proteins in the nervous system of Drosophila.”  Pongs O.et.al.   2456921
[4] “Multiple potassium-channel components are produced by alternative splicing at the Shaker locus in Drosophila.”  Schwarz T.L.et.al.   2448635
[5] “The genome sequence of Drosophila melanogaster.”  Adams M.D.et.al.   10731132
[6] “Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.”  Misra S.et.al.   12537572
[7] “A Drosophila full-length cDNA resource.”  Stapleton M.et.al.   12537569
[8] “Molecular characterization of Shaker, a Drosophila gene that encodes a potassium channel.”  Kamb A.et.al.   2440582
[9] “Multiple products of the Drosophila Shaker gene may contribute to potassium channel diversity.”  Kamb A.et.al.   3272175
[10] “Nervous system targets of RNA editing identified by comparative genomics.”  Hoopengardner B.et.al.   12907802
[11] “Reduced sleep in Drosophila Shaker mutants.”  Cirelli C.et.al.   15858564
[12] “Identification of N-glycosylated proteins from the central nervous system of Drosophila melanogaster.”  Koles K.et.al.   17893096
[13] “Three-dimensional structure of the S4-S5 segment of the Shaker potassium channel.”  Ohlenschlaeger O.et.al.   12023222
Structure:
1HO2   1HO7     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MAAVAGLYGL GEDRQHRKKQ QQQQQHQKEQ LEQKEEQKKI AERKLQLREQ QLQRNSLDGY 
61:	GSLPKLSSQD EEGGAGHGFG GGPQHFEPIP HDHDFCERVV INVSGLRFET QLRTLNQFPD 
121:	TLLGDPARRL RYFDPLRNEY FFDRSRPSFD AILYYYQSGG RLRRPVNVPL DVFSEEIKFY 
181:	ELGDQAINKF REDEGFIKEE ERPLPDNEKQ RKVWLLFEYP ESSQAARVVA IISVFVILLS 
241:	IVIFCLETLP EFKHYKVFNT TTNGTKIEED EVPDITDPFF LIETLCIIWF TFELTVRFLA 
301:	CPNKLNFCRD VMNVIDIIAI IPYFITLATV VAEEEDTLNL PKAPVSPQDK SSNQAMSLAI 
361:	LRVIRLVRVF RIFKLSRHSK GLQILGRTLK ASMRELGLLI FFLFIGVVLF SSAVYFAEAG 
421:	SENSFFKSIP DAFWWAVVTM TTVGYGDMTP VGVWGKIVGS LCAIAGVLTI ALPVPVIVSN 
481:	FNYFYHRETD QEEMQSQNFN HVTSCPYLPG TLGQHMKKSS LSESSSDMMD LDDGVESTPG 
541:	LTETHPGRSA VAPFLGAQQQ QQQPVASSLS MSIDKQLQHP LQQLTQTQLY QQQQQQQQQQ 
601:	QNGFKQQQQQ TQQQLQQQQS HTINASAAAA TSGSGSSGLT MRHNNALAVS IETDV