1.A.1.4.12 Synthetic light-sensitive K+ channel, BLINK2, of 406 aas and 2 TMSs with a P-loop between the two TMSs (residues 158 - 233). Residues 8 - 142 are derived from residues 403 - 537 of NPH1-1, a light-sensitive ser/thr protein kinase of the oat plant, Avena sativa ( acc # AAC05083); residues 143 - 234 are derived from residues 3 - 94 of the Paramecium bursaria Chlorella virus 1 (PBCV-1) K+ channel, Kcv1 (TC# 1.A.1.12.1); residues 235 - 404 derive from residues 506 - 675 of another K+ channel protein, KAT1 (TC# 1.A.1.12.1). BLINK1 has been used to manipulate stomatal kinetics to improve carbon assimilation, water use, and growth of A. thaliana (Papanatsiou et al. 2019).
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Accession Number: | AYS76116 |
Protein Name: | AYS76116.1 BLINK2 [synthetic construct] |
Length: | 407 |
Molecular Weight: | |
Species: | synthetic construct [32630] |
Number of TMSs: | 3 |
Substrate |
potassium(1+) |
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1: MGCTVSAELA TTLERIEKNF VITDPRLPDN PIIFASDSFL QLTEYSREEI LGRNCRFLQG
61: PETDRATVRK IRDAIDNQTE VTVQLINYTK SGKKFWNLFH LQPMRDQKGD VQYFIGVDLD
121: GTEHVRDAAE REGVMLIKKT AEVFSKFLTR TEPFMIHLFI LAMFVMIYKF FPGGFENNFS
181: VANPDKKASW IDCIYFGVTT HSTVGFGDIL PKTTGAKLCT IAHIVTVFFI VLTLDDSNTS
241: GHENRDFKSM GWEEWRDSRK DGYGLDVTNP TSDTALMDAI HKEDTEMVKK ILKEQKIERA
301: KVERSSSETA GRSYANDSSK KDPYCSSSNQ IIKPCKREEK RVTIHMMSES KNGKLILLPS
361: SIEELLRLAS EKFGGCNFTK ITNADNAEID DLDVIWDGDH LYFSDN