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1.A.79.1.1
The dsRNA transporter, SID-1 (Systematic RNA interference defective-1).  Sid1 forms a gated transmembrane channel (Shih and Hunter 2011).  It may function together with or be regulated by Sid-2, a metal-dependent nucleic acid binding protein (Q9GZC9) (McEwan et al. 2012), Sid-3, a tyrosyl protein kinase (Q10925), named Cdc-42-associated kinase, Ack, in mammals (Jose et al. 2012) and Sid-5 (Q19443) which co-localizes with RAB-7 (Q23146) and RLP-1 (Q11117).  Endocytosis may play a role in dsRNA uptake.  In Caenorhabditis elegans, inter-cellular transport of the small non-coding RNA causing systemic RNAi is mediated by the transmembrane protein SID1, encoded by the sid1 gene in the systemic RNAi defective (sid) loci. SID1 shares structural and sequence similarity with cholesterol uptake protein 1 (CHUP1) and is classified as a member of the ChUP family. Although systemic RNAi is not an evolutionarily conserved process, the sid gene products are found across the animal kingdom, suggesting the existence of other novel gene regulatory mechanisms mediated by small non-coding RNAs (Navratna et al. 2024). 

Accession Number:Q9GZC8
Protein Name:SID-1
Length:776
Molecular Weight:87931.00
Species:Caenorhabditis elegans [6239]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate double-stranded RNA

Cross database links:

RefSeq: NP_504372.2   
Entrez Gene ID: 178900   
KEGG: cel:C04F5.1    cel:C04F5.1   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005215 F:transporter activity
GO:0016246 P:RNA interference
GO:0005887 C:integral to plasma membrane
GO:0051033 F:RNA transmembrane transporter activity
GO:0033227 P:dsRNA transport

References (8)

[1] “Systemic RNAi in C. elegans requires the putative transmembrane protein SID-1.”  Winston W.M.et.al.   11834782
[2] “Genome sequence of the nematode C. elegans: a platform for investigating biology.”  The C. elegans sequencing consortiumet.al.   9851916
[3] “Transport of dsRNA into cells by the transmembrane protein SID-1.”  Feinberg E.H.et.al.   12970568
[4] “Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins.”  Kaji H.et.al.   17761667
[5] “Systemic RNAi in C. elegans requires the putative transmembrane protein SID-1.”  Winston W.M.et.al.   11834782
[6] “Genome sequence of the nematode C. elegans: a platform for investigating biology.”  The C. elegans sequencing consortiumet.al.   9851916
[7] “Transport of dsRNA into cells by the transmembrane protein SID-1.”  Feinberg E.H.et.al.   12970568
[8] “Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins.”  Kaji H.et.al.   17761667

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MIRVYLIILM HLVIGLTQNN STTPSPIITS SNSSVLVFEI SSKMKMIEKK LEANTVHVLR 
61:	LELDQSFILD LTKVAAEIVD SSKYSKEDGV ILEVTVSNGR DSFLLKLPTV YPNLKLYTDG 
121:	KLLNPLVEQD FGAHRKRHRI GDPHFHQNLI VTVQSRLNAD IDYRLHVTHL DRAQYDFLKF 
181:	KTGQTTKTLS NQKLTFVKPI GFFLNCSEQN ISQFHVTLYS EDDICANLIT VPANESIYDR 
241:	SVISDKTHNR RVLSFTKRAD IFFTETEISM FKSFRIFVFI APDDSGCSTN TSRKSFNEKK 
301:	KISFEFKKLE NQSYAVPTAL MMIFLTTPCL LFLPIVINII KNSRKLAPSQ SNLISFSPVP 
361:	SEQRDMDLSH DEQQNTSSEL ENNGEIPAAE NQIVEEITAE NQETSVEEGN REIQVKIPLK 
421:	QDSLSLHGQM LQYPVAIILP VLMHTAIEFH KWTTSTMANR DEMCFHNHAC ARPLGELRAW 
481:	NNIITNIGYT LYGAIFIVLS ICRRGRHEYS HVFGTYECTL LDVTIGVFMV LQSIASATYH 
541:	ICPSDVAFQF DTPCIQVICG LLMVRQWFVR HESPSPAYTN ILLVGVVSLN FLISAFSKTS 
601:	YVRFIIAVIH VIVVGSICLA KERSLGSEKL KTRFFIMAFS MGNFAAIVMY LTLSAFHLNQ 
661:	IATYCFIINC IMYLMYYGCM KVLHSERITS KAKLCGALSL LAWAVAGFFF FQDDTDWTRS 
721:	AAASRALNKP CLLLGFFGSH DLWHIFGALA GLFTFIFVSF VDDDLINTRK TSINIF