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1.A.8.10.1
Tonoplast intrinsic protein, TIPA or Tip3.1, of 268 aas and 6 TMSs.  Phylogenetic distribution, structure, transport dynamics, gating mechanism, sub-cellular localization, tissue-specific expression, and co-expression of TIPs have been reviewed to define their versatile role in plants (Sudhakaran et al. 2021). Based on the phylogenetic distribution, TIPs are classified into five distinct groups with aromatic-arginine (Ar/R) selectivity filters, typical pore-morphology, and tissue-specific gene expression patterns. The tissue-specific expression of TIPs is conserved among diverse plant species, especially for TIP3s, which are expressed exclusively in seeds. The solute specificities of TIPs plays a role in physiological processes like stomatal movement and vacuolar sequestration as well as in alleviating environmental stress. TIPs also play a role in growth and developmental processes like radicle protrusion, anther dehiscence, seed germination, cell elongation, and expansion. The gating mechanism of TIPs regulates the solute flow in response to external signals, which helps to maintain the physiological functions of the cell (Sudhakaran et al. 2021).

Accession Number:P26587
Protein Name:TIPA aka TIP3.1 aka AT1G73190 aka T18K17.14
Length:268
Molecular Weight:28308.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Vacuole membrane1 / Multi-pass membrane protein2
Substrate water

Cross database links:

Genevestigator: P26587
eggNOG: KOG0223
HEGENOM: HBG705794
RefSeq: NP_177462.1   
Entrez Gene ID: 843653   
Pfam: PF00230   
KEGG: ath:AT1G73190   

Gene Ontology

GO:0042807 C:central vacuole
GO:0016021 C:integral to membrane
GO:0009705 C:plant-type vacuole membrane
GO:0000326 C:protein storage vacuole
GO:0005215 F:transporter activity
GO:0006914 P:autophagy
GO:0055085 P:transmembrane transport

References (5)

[1] “Vegetative and seed-specific forms of tonoplast intrinsic protein in the vacuolar membrane of Arabidopsis thaliana.”  Hoefte H.R.et.al.   16668923
[2] “Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.”  Theologis A.et.al.   11130712
[3] “Empirical analysis of transcriptional activity in the Arabidopsis genome.”  Yamada K.et.al.   14593172
[4] “Characterization of a new vacuolar membrane aquaporin sensitive to mercury at a unique site.”  Daniels M.J.et.al.   8624437
[5] “From genome to function: the Arabidopsis aquaporins.”  Quigley F.et.al.   11806824

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MATSARRAYG FGRADEATHP DSIRATLAEF LSTFVFVFAA EGSILSLDKL YWEHAAHAGT 
61:	NTPGGLILVA LAHAFALFAA VSAAINVSGG HVNPAVTFGA LVGGRVTAIR AIYYWIAQLL 
121:	GAILACLLLR LTTNGMRPVG FRLASGVGAV NGLVLEIILT FGLVYVVYST LIDPKRGSLG 
181:	IIAPLAIGLI VGANILVGGP FSGASMNPAR AFGPALVGWR WHDHWIYWVG PFIGSALAAL 
241:	IYEYMVIPTE PPTHHAHGVH QPLAPEDY