1.A.9.4.5
Glutamate-gated chloride channel of 448 aas, GluCl.  A point mutation, A309V in TMS 3, renders the protein and the organism > 11,000-fold resistant to abamectin, an insecticide of this moth, which is a global pest of cruciferous vegetables (Wang et al. 2015). Both A309V and G315E mutations contribute to target-site resistance to abamectin (Wang et al. 2017). Fluralaner (Bravecto) is an isoxazoline ectoparasiticide which potently inhibits GABA-gated chloride channels (GABACls) and less potently glutamate-gated chloride channels (GluCls) in insects. The amino acid, Leu315, in Musca (fly) GluCls is important in determining the selectivity of fluralaner and ivermectin which react in opposite ways (Nakata et al. 2017).