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1.B.11.2.1
Type π fimbrial usher, PapC. The crystal structure of the PapC usher translocation domain has been solved (Daniels and Normark, 2008; Remaut et al., 2008).  The crystal structure of the full-length PapC usher in complex with its cognate PapDG chaperone-subunit complex in a pre-activation state has been solved.  This elucidated the molecular details of how the usher is specifically engaged by allosteric interactions with its substrate, preceding activation, and how the usher facilitates the transfer of subunits from the amino-terminal periplasmic domain to the CTDs during pilus assembly (Omattage et al. 2018).

Accession Number:P07110
Protein Name:PapC
Length:836
Molecular Weight:91509.00
Species:Escherichia coli [562]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell outer membrane1 / Multi-pass membrane protein2
Substrate

Cross database links:

Pfam: PF00577   

Gene Ontology

GO:0009279 C:cell outer membrane
GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity
GO:0006810 P:transport

References (6)

[1] “Nucleotide sequence, regulation and functional analysis of the papC gene required for cell surface localization of Pap pili of uropathogenic Escherichia coli.”  Norgren M.et.al.   2897064
[2] “Horizontal gene transfer of the Escherichia coli pap and prs pili operons as a mechanism for the development of tissue-specific adhesive properties.”  Marklund B.-I.et.al.   1357526
[3] “Bacterial outer membrane ushers contain distinct targeting and assembly domains for pilus biogenesis.”  Thanassi D.G.et.al.   12399496
[4] “PapD, a periplasmic transport protein in P-pilus biogenesis.”  Lindberg F.et.al.   2572580
[5] “Chaperone-assisted self-assembly of pili independent of cellular energy.”  Jacob-Dubuisson F.et.al.   7909802
[6] “The PapC usher forms an oligomeric channel: implications for pilus biogenesis across the outer membrane.”  Thanassi D.G.et.al.   9501230
Structure:
2VQI   3FIP   2KT6   6CD2     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MKDRIPFAVN NITCVILLSL FCNAASAVEF NTDVLDAADK KNIDFTRFSE AGYVLPGQYL 
61:	LDVIVNGQSI SPASLQISFV EPALSGDKAE KKLPQACLTS DMVRLMGLTA ESLDKVVYWH 
121:	DGQCADFHGL PGVDIRPDTG AGVLRINMPQ AWLEYSDATW LPPSRWDDGI PGLMLDYNLN 
181:	GTVSRNYQGG DSHQFSYNGT VGGNLGPWRL RADYQGSQEQ SRYNGEKTTN RNFTWSRFYL 
241:	FRAIPRWRAN LTLGENNINS DIFRSWSYTG ASLESDDRML PPRLRGYAPQ ITGIAETNAR 
301:	VVVSQQGRVL YDSMVPAGPF SIQDLDSSVR GRLDVEVIEQ NGRKKTFQVD TASVPYLTRP 
361:	GQVRYKLVSG RSRGYGHETE GPVFATGEAS WGLSNQWSLY GGAVLAGDYN ALAAGAGWDL 
421:	GVPGTLSADI TQSVARIEGE RTFQGKSWRL SYSKRFDNAD ADITFAGYRF SERNYMTMEQ 
481:	YLNARYRNDY SSREKEMYTV TLNKNVADWN TSFNLQYSRQ TYWDIRKTDY YTVSVNRYFN 
541:	VFGLQGVAVG LSASRSKYLG RDNDSAYLRI SVPLGTGTAS YSGSMSNDRY VNMAGYTDTF 
601:	NDGLDSYSLN AGLNSGGGLT SQRQINAYYS HRSPLANLSA NIASLQKGYT SFGVSASGGA 
661:	TITGKGAALH AGGMSGGTRL LVDTDGVGGV PVDGGQVVTN RWGTGVVTDI SSYYRNTTSV 
721:	DLKRLPDDVE ATRSVVESAL TEGAIGYRKF SVLKGKRLFA ILRLADGSQP PFGASVTSEK 
781:	GRELGMVADE GLAWLSGVTP GETLSVNWDG KIQCQVNVPE TAISDQQLLL PCTPQK