1.B.165.1.1
AvrE (DspE) of 1838 aas and a beta (β) structure after secretion from the bacteriial cytoplasm via a type III protein secretion system (TC# 3.A.6) into the host cell and insertion into the plasma membrane of the plant cell where it transports metabolites, water and small molecules such as fluorescene dyes (Nomura et al. 2023). DspE in Xenopus oocytes resulted in inward and outward currents,
permeability to water and osmolarity-dependent oocyte swelling and
bursting. Liposome reconstitution confirmed that the DspE channel alone
is sufficient to allow the passage of small molecules such as
fluorescein dye. Targeted screening of chemical blockers based on the
predicted pore size (15-20 Å) of the DspE channel identified
polyamidoamine dendrimers as inhibitors of the DspE/AvrE channels.
Notably, polyamidoamines broadly inhibit AvrE and DspE virulence
activities in Xenopus oocytes and during E. amylovora and P. syringae infections.
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| Accession Number: | WP_248401767.1 |
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| Protein Name: | WP_248401767.1 AvrE-family type 3 secretion system effector [Erwinia amylovora] |
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| Length: | 1839 |
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| Molecular Weight: | |
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| Species: | Erwinia amylovora [552] |
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| Substrate |
water |
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1: MELKSLGTEH KAAVHTAAHN PVGHGVALQQ GSSSSSPQNA AASLAAEGKN RGKMPRIHQP
61: STAADGISAA HQQKKSFSLR GCLGTKKFSR SAPQGQPGTT HSKGATLRDL LARDDGETQH
121: EAAAPDAARL TRSGGVKRRN MDDMAGRPMV KGGSGEDKVP TQQKRHQLNN FGQMRQTMLS
181: KMAHPASANA GDRLQHSPPH IPGSHHEIKE EPVGSTSKAT TAHADRVEIA QEDDDSEFQQ
241: LHQQRLARER ENPPQPPKLG VATPISARFQ PKLTAVAESV LEGTDTTQSP LKPQSMLKGS
301: GAGVTPLAVT LDKGKLQLAP DNPPALNTLL KQTLGKDTQH YLAHHASSDG SQHLLLDNKG
361: HLFDIKSTAT SYSVLHNSHP GEIKGKLAQA GTGSVSVDGK SGKISLGSGT QSHNKTMLSQ
421: PGEAHRSLLT GIWQHPAGAA RPQGESIRLH DDKIHILHPE LGVWQSADKD THSQLSRQAD
481: GKLYALKDNR TLQNLSDNKS SEKLVDKIKS YSVDQRGQVA ILTDTPGRHK MSIMPSLDAS
541: PESHISLSLH FADAHQGLLH GKSELEAQSV AISHGRLVVA DSEGKLFSAA IPKQGDGNEL
601: KMKAMPQHAL DEHFGHDHQI SGFFHDDHGQ LNALVKNNFR QQHACPLGND HQFHPGWNLT
661: DALVIDNQLG LHHTNPEPHE ILDMGHLGSL ALQEGKLHYF DQLTKGWTGA ESDCKQLKKG
721: LDGAAYLLKD GEVKRLNINQ STSSIKHGTE NVFSLPHVRN KPEPGDALQG LNKDDKAQAM
781: AVIGVNKYLA LTEKGDIRSF QIKPGTQQLE RPAQTLSREG ISGELKDIHV DHKQNLYALT
841: HEGEVFHQPR EAWQNGAESS SWHKLALPQS ESKLKSLDMS HEHKPIATFE DGSQHQLKAG
901: GWHAYAAPER GPLAVGTSGS QTVFNRLMQG VKGKVIPGSG LTVKLSAQTG GMTGAEGRKV
961: SSKFSERIRA YAFNPTMSTP RPIKNAAYAT QHGWQGREGL KPLYEMQGAL IKQLDAHNVR
1021: HNAPQPDLQS KLETLDLGEH GAELLNDMKR FRDELEQSAT RSVTVLGQHQ GVLKSNGEIN
1081: SEFKPSPGKA LVQSFNVNRS GQDLSKSLQQ AVHATPPSAE SKLQSMLGHF VSAGVDMSHQ
1141: KGEIPLGRQR DPNDKTALTK SRLILDTVTI GELHELADKA KLVSDHKPDA DQIKQLRQQF
1201: DTLREKRYES NPVKHYTDMG FTHNKALEAN YDAVKAFINA FKKEHHGVNL TTRTVLESQG
1261: SAELAKKLKN TLLSLDSGES MSFSRSYGGG VSTVFVPTLS KKVPVPVIPG AGITLDRAYN
1321: LSFSRTSGGL NVSFGRDGGV SGNIMVATGH DVMPYMTGKK TSAGNASDWL SAKHKISPDL
1381: RIGAAVSGTL QGTLQNSLKF KLTEDELPGF IHGLTHGTLT PAELLQKGIE HQMKQGSKLT
1441: FSVDTSANLD LRAGINLNED GSKPNGVTAR VSAGLSASAN LAAGSRERST TSGQFGSTTS
1501: ASNNRPTFLN GVGAGANLTA ALGVAHSSTH EGKPVGIFPA FTSTNVSAAL ALDNRTSQSI
1561: SLELKRAEPV TSNDISELTS TLGKHFKDSA TTKMLAALKE LDDAKPAEQL HILQQHFSAK
1621: DVVGDERYEA VRNLRKLVIR QQAADSHSME LGSASHSTTY NNLSRINNDG IVELLHKHFD
1681: AALPASSAKR LGEMMNNDPA LKDIIKQLQS TPFSSASVSM ELKDGLREQT EKAILDGKVG
1741: REEVGVLFQD RNNLRVKSVS VSQSVSKSEG FNTPALLLGT SNSAAMSMER NIGTINFKYG
1801: QDQNTPRRFT LEGGIAQANP QVASALTDLK KEGLEMKS