TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


1.B.18.3.1
OMA component of the group 1 K30 capsular polysaccharide, colanic acid, export system, Wza (Reid and Whitfield, 2005) (outer membrane porin constituent). The 3-d structure of the Wza/Wzc complex has been solved by Collins et al. (2007). It spans the periplasm, comprising a central constituent for complex formation. The x-ray structure shows an integral outer membrane transmembrane α-helical barrel with a large central cavity, similar to the secretin protein, PilQ (1.B.22.2.1) (Collins and Derrick, 2007).  Wza is an octameric α-helical outer membrane channel that directly exports nascent capsular polysaccharide chains through the Wza portal (Nickerson et al. 2014). Peptides based on the C-terminal D4 domain of Wza form transmembrane, ion conducting α-helical barrels. The helix barrel contains eight D4 peptides arranged in parallel (Mahendran et al. 2017).

Accession Number:P0A930
Protein Name:Putative polysaccharide export protein Wza aka B2062
Length:379
Molecular Weight:41910.00
Species:Escherichia coli [83333]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell outer membrane1 / Multi-pass membrane protein2
Substrate

Cross database links:

RefSeq: AP_002662.1    NP_416566.1   
Entrez Gene ID: 946558   
Pfam: PF02563    PF10531   
BioCyc: EcoCyc:G7107-MONOMER    ECOL168927:B2062-MONOMER   
KEGG: ecj:JW2047    eco:b2062   

Gene Ontology

GO:0009279 C:cell outer membrane
GO:0005886 C:plasma membrane
GO:0046930 C:pore complex
GO:0015159 F:polysaccharide transmembrane transporter ac...
GO:0006811 P:ion transport
GO:0000271 P:polysaccharide biosynthetic process
GO:0015774 P:polysaccharide transport

References (4)

[1] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[2] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[3] “Identification of the promoter region for the colanic acid polysaccharide biosynthetic genes in Escherichia coli K-12.”  Stout V.et.al.   8763957
[4] “Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid.”  Stevenson G.et.al.   8759852

External Searches:

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence
1:	MMKSKMKLMP LLVSVTLISG CTVLPGSNMS TMGKDVIKQQ DADFDLDKMV NVYPLTPRLI 
61:	DQLRPRPNVA RPNMTLESEI ANYQYRVGPG DVLNVTVWDH PELTTPAGQY RSSSDTGNWV 
121:	QPDGTMFYPY IGKVHVVGKT LAEIRSDITG RLATYIADPQ VDVNIAAFRS QKAYISGQVN 
181:	KSGQQAITNV PLTILDAINA AGGLTDTADW RNVVLTHNGR EERISLQALM QNGDLNQNRL 
241:	LYPGDILYVP RNDDLKVFVM GEVKKQSTLK MDFSGMTLTE ALGNAEGIDM TTSNASGIFV 
301:	IRPLKGEGGR NGKIANIYQL DMSDATSLVM ATEFRLQPYD VVYVTTAPVS RWNRLINQLL 
361:	PTISGVRYMT DTASDIHNW