Search TCDB: (?)
TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


1.B.25.1.1
OprD2; OccD1; porin D transports cationic amino acids, peptides and other compounds: lysine, arginine, histidine, ornithine, basic di- and tri-peptides, and cationic antibiotics such as imipenem (n-formimidoylthienamycin) and other penems and carbapenems (Tamber et al., 2006).  The 3-d structure and drugs transported are known (4FOZ; Parkin and Khalid 2014).  OprD is the vitronectin receptor. Vitronectin enhances P. aeruginosa adhesion to host epithelial cells and thereby enhances virulence (Paulsson et al. 2015).  Loss promotes carbapenem resistance (Shen and Fang 2015; Cavalcanti et al. 2015). Loss results in resistance to  meropenem (Fluit et al. 2019).  Carbapenem resistance in difficult-to-treat P. aeruginosa  strains can be mediated by loss or reduction of the OprD porin (Do Rego and Timsit 2023).

Accession Number:P32722
Protein Name:PORD aka OprD2 aka PA0958
Length:443
Molecular Weight:48360.00
Species:Pseudomonas aeruginosa [287]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell outer membrane1 / Multi-pass membrane protein2
Substrate lysine, ornithine, arginine, histidine, imipenem, penems, meropenem, 2-(Biaryl)carbapenems

Cross database links:

DIP: DIP-29627N
RefSeq: NP_249649.1   
Entrez Gene ID: 881970   
Pfam: PF03573   
BioCyc: PAER208964:PA0958-MONOMER   
KEGG: pae:PA0958   

Gene Ontology

GO:0045203 C:integral to cell outer membrane
GO:0005886 C:plasma membrane
GO:0046930 C:pore complex
GO:0015288 F:porin activity
GO:0008236 F:serine-type peptidase activity
GO:0015802 P:basic amino acid transport
GO:0006811 P:ion transport

References (7)

[1] “Nucleotide sequence of the protein D2 gene of Pseudomonas aeruginosa.”  Yoneyama H.et.al.   1339257
[2] “Analysis of two gene regions involved in the expression of the imipenem-specific, outer membrane porin protein OprD of Pseudomonas aeruginosa.”  Huang H.et.al.   1427017
[3] “Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen.”  Stover C.K.et.al.   10984043
[4] “Two-dimensional polyacrylamide gel electrophoresis isolation and microsequencing of Pseudomonas aeruginosa proteins.”  Michea-Hamzehpour M.et.al.   8012497
[5] “Protein D2 channel of the Pseudomonas aeruginosa outer membrane has a binding site for basic amino acids and peptides.”  Trias J.et.al.   2118530
[6] “Protein D2 porin of the Pseudomonas aeruginosa outer membrane bears the protease activity.”  Yoshihara E.et.al.   8843159
[7] “Identification of the catalytic triad of the protein D2 protease in Pseudomonas aeruginosa.”  Yoshihara E.et.al.   9636669
Structure:
2ODJ   3SY7   4FOZ     

External Searches:

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence 
1:	MKVMKWSAIA LAVSAGSTQF AVADAFVSDQ AEAKGFIEDS SLDLLLRNYY FNRDGKSGSG 
61:	DRVDWTQGFL TTYESGFTQG TVGFGVDAFG YLGLKLDGTS DKTGTGNLPV MNDGKPRDDY 
121:	SRAGGAVKVR ISKTMLKWGE MQPTAPVFAA GGSRLFPQTA TGFQLQSSEF EGLDLEAGHF 
181:	TEGKEPTTVK SRGELYATYA GETAKSADFI GGRYAITDNL SASLYGAELE DIYRQYYLNS 
241:	NYTIPLASDQ SLGFDFNIYR TNDEGKAKAG DISNTTWSLA AAYTLDAHTF TLAYQKVHGD 
301:	QPFDYIGFGR NGSGAGGDSI FLANSVQYSD FNGPGEKSWQ ARYDLNLASY GVPGLTFMVR 
361:	YINGKDIDGT KMSDNNVGYK NYGYGEDGKH HETNLEAKYV VQSGPAKDLS FRIRQAWHRA 
421:	NADQGEGDQN EFRLIVDYPL SIL