1.C.107.1.1
Insecticidal toxin complex (TC) component, TcaA (1095 aas) (may mediate toxin-C internalization)(Landsberg et al., 2011). The TcdA1 prepore assembles as a pentamer forming an α-helical,
vuvuzela-shaped channel less than 1.5 nanometres in diameter surrounded
by a large outer shell (Gatsogiannis et al. 2013). Membrane insertion is triggered not only at low
pH as expected, but also at high pH, explaining Tc action directly
through the midgut of insects. Comparisons with structures of the TcdA1
pore inserted into a membrane and in complex with TcdB2 and TccC3 reveal
large conformational changes during membrane insertion, suggesting a novel syringe-like mechanism of protein translocation (Gatsogiannis et al. 2013). P. luminescens is a nematode symbiont and an insect pathogen. The toxin, TcC, of the tripartite toxin complex, is an ADP ribosyl transferase causing actin clustering, defects in phagocytosis and cell dealth.
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| Accession Number: | Q66PW7 |
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| Protein Name: | TcaA |
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| Length: | 1095 |
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| Molecular Weight: | 119513.00 |
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| Species: | Photorhabdus luminescens subsp. luminescens [171439] |
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| Substrate |
toxin |
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1: MVTVMQNKVS FLSGTSEQPL LDAGYQNVFD IASISRAAFV QSVPTLPVKE AHTVYRQARQ
61: RAENLKSLYR AWQLRQEPVI KGLTKLNAQA NVSVLQDALV ENIGGDGDFS DLMNRASQYA
121: DAASIQSLFS PGRYATALYK VAKDLHKSDS SLHIDNRRAD LKDLILNETM MNKEVTSLDI
181: LLDILQKGSK DITELSSAFF PMTLPYDDHL SQIDSALSAQ ARTLNGVWNT LTDTTAQAVS
241: EQAGNTNTLK LFAAQNDNQD TFFAGNTFYF KAVGFGEQPM VYPSQYTSGS GIVGAQLIAG
301: NPDQAAAAIV APLQLTWSMA KQCYYLGAPD GTTMGDGNVL TGCFLRGNSP TNPDQNGIFA
361: QVANKSGSTQ PISGFHLPVT LERSEDKDKY YLKTEQGYIA VGSSGQSNWK NALVINGTKD
421: NGLLLTFCSD SSGTPTNPDD VIPPAINDIP SPPARETLSL TPVSYQLMTN LAPTEDDITH
481: HYGLGGSSLR DATSSASALT TALNSIETFC EKTRINFNQL MDLTAQQSYS QSSVDAKAAS
541: RYVRFGETAA TRVNVYGAAY LNSTLAAAAD GQYLWIQTDG KSLNFTDDTV VALAGRAEKL
601: VRLSSQTGLS FEELDWLIVN ASRSVPDHHD KIVLDKPVLE ALAEYVSLKQ RYGLDANTFV
661: TFISAVNPYT PDQTPSFYET TFRSADGNHV IALGTAVKYA ENEQDELSTI CCKALGVTSD
721: EFFRIGRYCF GNAGSFTLDE YTASQLYRFG AIPRLFGLTF AQAEILWRLM EGGKDILLQQ
781: LGQAKSLQPL AILRRTEQVL DWMSSVNLSL TYLQGMVSTQ WSGTATAEMF NFLENVCDSV
841: NSQTAAKETM DSALQQKVLR ALSAGFGIKS NVMGIVTFWL EKITASSGNP FTLANYWHDI
901: QTLFSHNNAT LDNLQTDTFL VIATQQLSQL VLIVKWLSLT EQDLQLLTTY PERLLNGITH
961: VPAPNPELLL TLSRFKQWQT QVTVSRDEAM RCFDQLNAEG MTADSAASLI ATLHEMDKGT
1021: VAQVNTLLSG ENNWPKSFTS LWQLLTWLRV GQSLNVGSTT LGNLLAMMQA DPAAESSALL
1081: ASVAQNLSAA ISNRQ