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1.C.2.1.2
Pesticide crystal protein Cry4Ba (δ-endotoxin) (1136aas). Cadherin AgCad1 is the receptor for Cry4Ba (Hua et al., 2008).  Asn183 in TMS5 is essential for oligomerizatioin of the protein in the midgut membrane of the insect, and therefore for pore formation and toxicity (Likitvivatanavong et al. 2006).  The complete structure of a functional form of the Bacillus thuringiensis Cry4Ba delta-endotoxinhas been solved with nsight into the toxin-induced transmembrane pore architecture (Thamwiriyasati et al. 2022). The 2.0 Å crystal structure revealed a wedge-shaped arrangement of three domains: a well-defined N-terminal domain of eight alpha-helices responsible for pore formation, a three-beta-sheet prism displaying two functional motifs and a C-terminal beta-sandwich domain. Two conserved side-chains-Asn(166) and Tyr(170) in the α4-α5 loop were found to interact directly with phospholipid head groups, leading to pore opening and stability (Thamwiriyasati et al. 2022).

Accession Number:P05519
Protein Name:Cry4Ba
Length:1136
Molecular Weight:127764.00
Species:Bacillus thuringiensis subsp. israelensis [1430]
Location1 / Topology2 / Orientation3: Mitochondrion inner membrane1 / Multi-pass membrane protein2
Substrate molecule

Cross database links:

RefSeq: YP_001573790.1   
Entrez Gene ID: 5759934   
Pfam: PF03944    PF00555    PF03945   

Gene Ontology

GO:0005102 F:receptor binding
GO:0006952 P:defense response
GO:0009405 P:pathogenesis
GO:0030435 P:sporulation resulting in formation of a cel...

References (4)

[1] “The complete nucleotide sequence of a 130 kDa mosquito-larvicidal delta-endotoxin gene of Bacillus thuringiensis var. israelensis.”  Tungpradubkul S.et.al.   2831510
[2] “Common features of Bacillus thuringiensis toxins specific for Diptera and Lepidoptera.”  Chungjatupornchai W.et.al.   2833395
[3] “Nucleotide sequence of the gene coding for a 130-kDa mosquitocidal protein of Bacillus thuringiensis israelensis.”  Yamamoto T.et.al.   2901387
[4] “Cloning and expression of 130-kd mosquito-larvicidal delta-endotoxin gene of Bacillus thuringiensis var. israelensis in Escherichia coli.”  Angsuthanasombat C.et.al.   2890080
Structure:
1W99   2J35   4MOA     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MNSGYPLAND LQGSMKNTNY KDWLAMCENN QQYGVNPAAI NSSSVSTALK VAGAILKFVN 
61:	PPAGTVLTVL SAVLPILWPT NTPTPERVWN DFMTNTGNLI DQTVTAYVRT DANAKMTVVK 
121:	DYLDQYTTKF NTWKREPNNQ SYRTAVITQF NLTSAKLRET AVYFSNLVGY ELLLLPIYAQ 
181:	VANFNLLLIR DGLINAQEWS LARSAGDQLY NTMVQYTKEY IAHSITWYNK GLDVLRNKSN 
241:	GQWITFNDYK REMTIQVLDI LALFASYDPR RYPADKIDNT KLSKTEFTRE IYTALVESPS 
301:	SKSIAALEAA LTRDVHLFTW LKRVDFWTNT IYQDLRFLSA NKIGFSYTNS SAMQESGIYG 
361:	SSGFGSNLTH QIQLNSNVYK TSITDTSSPS NRVTKMDFYK IDGTLASYNS NITPTPEGLR 
421:	TTFFGFSTNE NTPNQPTVND YTHILSYIKT DVIDYNSNRV SFAWTHKIVD PNNQIYTDAI 
481:	TQVPAVKSNF LNATAKVIKG PGHTGGDLVA LTSNGTLSGR MEIQCKTSIF NDPTRSYGLR 
541:	IRYAANSPIV LNVSYVLQGV SRGTTISTES TFSRPNNIIP TDLKYEEFRY KDPFDAIVPM 
601:	RLSSNQLITI AIQPLNMTSN NQVIIDRIEI IPITQSVLDE TENQNLESER EVVNALFTND 
661:	AKDALNIGTT DYDIDQAANL VECISEELYP KEKMLLLDEV KNAKQLSQSR NVLQNGDFES 
721:	ATLGWTTSDN ITIQEDDPIF KGHYLHMSGA RDIDGTIFPT YIFQKIDESK LKPYTRYLVR 
781:	GFVGSSKDVE LVVSRYGEEI DAIMNVPADL NYLYPSTFDC EGSNRCETSA VPANIGNTSD 
841:	MLYSCQYDTG KKHVVCQDSH QFSFTIDTGA LDTNENIGVW VMFKISSPDG YASLDNLEVI 
901:	EEGPIDGEAL SRVKHMEKKW NDQMEAKRSE TQQAYDVAKQ AIDALFTNVQ DEALQFDTTL 
961:	AQIQYAEYLV QSIPYVYNDW LSDVPGMNYD IYVELDARVA QARYLYDTRN IIKNGDFTQG 
1021:	VMGWHVTGNA DVQQIDGVSV LVLSNWSAGV SQNVHLQHNH GYVLRVIAKK EGPGNGYVTL 
1081:	MDCEENQEKL TFTSCEEGYI TKTVDVFPDT DRVRIEIGET EGSFYIESIE LICMNE