1.D.191.  The Engineered Holin-based Nanopore (EHN) Family

Nanopores have been engineered by recombining the transmembrane module of the S²¹68 holin with different ring-shaped oligomeric protein structures that feature defined hexa-, hepta-, and octameric geometries. Library screening highlighted substantial plasticity in the ability of the S²¹68 transmembrane module to oligomerize in alternative geometries, while the functional properties of the resultant nanopores could be fine-tuned through the identity of the connecting linkers (Weber et al. 2022).