1.D.231. The Artificial Liposome Compartment with Size Exclusion Molecular Transport (ALC-SET) Family
Efficient material production and diagnostics require features required for an artificial compartment are that it isolates the interior from the external environment while being further functionalized to control the transport of target chemicals to regulate the interior concentration of both substrate and reaction products. Zhang et al. 2023 have constructed an artificial compartment with a size-selective molecular transport function by using a DNA origami-guided liposome prepared by modifying the method reported by Perrault et al. This completely isolates the liposome interior, including the DNA origami skeleton, from the external environment and allows the assembly of a defined number of molecules of interest inside and/or outside the compartment. By incorporating a bacterial membrane protein, OmpF of E. coli into the liposome, the resulting artificial compartment transports only the molecule of interest with a molecular weight below 600 Da from the external environment into the interior of the compartment.