1.I.1.1.2
Fungal Nuclear Pore Complex (NPC) with 29 components. Stuwe et al. 2015 presented
the reconstitution of the ~425-kilodalton inner ring
complex (IRC), which forms the central transport channel and diffusion
barrier of the NPC, revealing its interaction network and equimolar
stoichiometry. The Nsp1•Nup49•Nup57 channel nucleoporin heterotrimer
(CNT) attaches to the IRC solely through the adaptor nucleoporin Nic96.
The CNT•Nic96 structure reveals that Nic96 functions as an assembly
sensor that recognizes the three-dimensional architecture of the CNT,
thereby mediating the incorporation of a defined CNT state into the NPC.
They proposed that the IRC adopts a relatively rigid scaffold that
recruits the CNT to primarily form the diffusion barrier of the NPC,
rather than enabling channel dilation (Stuwe et al. 2015 presented
the reconstitution of the ~425-kilodalton inner ring
complex (IRC), which forms the central transport channel and diffusion
barrier of the NPC, revealing its interaction network and equimolar
stoichiometry. The Nsp1•Nup49•Nup57 channel nucleoporin heterotrimer
(CNT) attaches to the IRC solely through the adaptor nucleoporin Nic96.
The CNT•Nic96 structure reveals that Nic96 functions as an assembly
sensor that recognizes the three-dimensional architecture of the CNT,
thereby mediating the incorporation of a defined CNT state into the NPC.
They proposed that the IRC adopts a relatively rigid scaffold that
recruits the CNT to primarily form the diffusion barrier of the NPC,
rather than enabling channel dilation (Stuwe et al. 2015).
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| Accession Number: | G0SBS8 |
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| Protein Name: | Nucleoporin NUP159 |
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| Length: | 1481 |
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| Molecular Weight: | 156196.00 |
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| Species: | Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) [759272] |
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| Number of TMSs: | 1 |
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| Location1 / Topology2 / Orientation3: |
Nucleus1 |
|---|
| Substrate |
protein polypeptide chain, (ribonucleotide)n+m |
|---|
1: MAFSFGNAGG GGGGVTQGKD LEVIQTEGLG FLALAGDAKV QLTSKWSPPP APTASLLSIA
61: SRKGLVAAAG PDAVHVATTE SVRKAFLAEK NGDSEVRPFN PEAKLPLPLR ISQLAFTADE
121: QYLVLSAETG GGLRAYDVNS LTQGNTQSAF ELATNGETLR QLAPNPMPES AAFCAIVTTN
181: GNLYMANLAE RTLVSGPNGP TLRSQVSCAA WSTKGKQLVA GMADGSIYQM TPDGTEKAHI
241: PKPPNLGDYH VSSVVWLENN VFLTIHNPTN STNPDDKTVY HVITRQQSSG SPPNFTFQKL
301: NDPVEPFVAD KTPHHTVLRL KDFPPNLQDL LLVSSTAVET IGLLTRSKTP LATDKPADAI
361: TNVFTTTELA DDSRRAQLPM SEDMMETYPI GVALDLSSKE KVYKPIPTDE EIEYSPGPLP
421: GLWVLNNEGV LASWWVVYNE SIRAGTTYSG IGGSSEVIPA SPAPALASST APVPAFASPV
481: SKPTFGSPSP ATPAFGGPSA LGTKASPWAT AGGAASSTPT FGQPSFGKPA APAFGQASFP
541: GLGQKVSPWA TGSTTSAAPA FGQSGFASAG TAPGKVFGSS FTAPSSGGFA SFATKSGFAS
601: LSAPSGGSSI FSSKPGAPLT SAAPEVSMDT DTAFPPPSTK TDKPAFGSSP FVLGSTFKAD
661: PTAAHDIEKP KEGESKSLFD TGFGLSLEDA AKQPASAAES KDEEMRSTTP PLPPPTETKP
721: KSIFESTTPT TTPAPQKFEF KTTTPSGFST LLGSTKPVAS SMPNIFATPK PTSAEKPKSI
781: FDTLKPKEES KENLLKASEP PLPPDTTSKA VFQPGSSSSE SAESSPGAAA KAAFKVGNDE
841: TPKPQKELAP KPEAVPLPPD FVKAKPKTEA KETKAEEPAV SPNLPVKPLA KKAEPIPAVP
901: ESASEEEQGQ AEEEEAESGE EEEEEEEEGE GEEEEEEEEE EEEEEEEGEE GEEQSEAGSE
961: GSGVDVAKDL SPTAKFGSMT PGYTPHTSLG GMAESTFSTI SRSEVAEQSR PLFGEITKNA
1021: PPLFPAAGPL PVSPRSPSPV RGVVRSSILR PTETPRPVDT TPVPSRKGLL QKTASFGMST
1081: GQKPAVDPNV KAQRKLAEKL KAEEQVLVDP EDEGIQQILQ SKVEPTLRMN EFLAVDTKLA
1141: PMKPGRDDVP NACETLWRDI NRMIDRLGLN SRSLQSFILG HTSHGKPGGR QKDDLEKPDD
1201: WVLIEAYDLG DMIDNKLARE LEAGRIKDVE GTMAAIHNLG RDLAKLRAKE EDLRKLFNAQ
1261: VDPDQIALTK ALPLSAEQLA QQNELRRSYA SFSKLLTEAE EALTVLKAKL ASANAARGRR
1321: GAGAAQVPTV DAIIRTINKM TSMAEKRSGD IDVLESQMRK LRIGSLGPAG TPNGNGVGAG
1381: TVVPATPGGG GRSRESSPFV TPQSSRRAMF MSPGSVGTAT PRGLLAATGT PSPTKKKLSM
1441: YTAEEKRELR AREAKRKATL RMLRESLARV GPNVVRLRDD D