1.N.3.1.6
Hapless 2, HAP2 or GCS1 of 1139 aas and 1 TMS. HAP2, an Arabidopsis sterility protein, localizes at the fusion site of Chlamydomonas minus gametes and mediates formation of tight prefusion membrane attachments with their respective
gamete partners (Fédry et al. 2017). Membrane dye experiments show that HAP2 is essential for membrane merger (Liu et al. 2008). This protein may be essential for gamete fusion in animals, plants, fungi and protists (Ning et al. 2013). X-ray crystallographic studies reveal homology to class II viral membrane fusion proteins. Targeting the segment corresponding to the fusion loop
by mutagenesis or by antibodies blocks gamete fusion (Fédry et al. 2017).
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| Accession Number: | A4GRC6 |
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| Protein Name: | Hapless 2 |
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| Length: | 1139 |
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| Molecular Weight: | 115204.00 |
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| Species: | Chlamydomonas reinhardtii [3055] |
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| Number of TMSs: | 1 |
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| Location1 / Topology2 / Orientation3: |
Cell membrane1 / Single-pass type I membrane protein2 |
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| Substrate |
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1: MCRAIAVALI VYLAQHYILA HAEVIASGRL EKCVVDGVTE ELDCQEKVVV TLTVGNGQSL
61: QTEALEFSLS CLNSPDGRCP CSCSAADPTC ACRDLAAPLR VSLTKSPLWA SYPLQYLSSF
121: NWKPLEVILR PSNKVCKDGD WEDSPTCGWF SQGGVRVADS QGFCCECSSS QVWDDTFGSS
181: KERTRANLDC DFWSDPLDIL IGRKPVSAHC LTFDPQWYSG YELGAASLQF EIAITVEVPT
241: APSPTTATTS ATPRTNNSSS ANSTNSTNSP APQFLSPPAP STREVLHLGP SVPLASSASR
301: LLSAKLLGDL AMYTQLPAIS NQVLMVPQPP AAAAATGSPL DATLATNRSA WMLLDKTMLS
361: MDGLACDKVG TGFSAFRYQP SGCGRAPQAC LSGQLKDLWE ADLARIADGR VPLYMITRFT
421: GGSDTTLQSF SGGPLSFALP VTSHSQSLVT LSVAADGVRL VTNRSPGKIT GAAVCRFAGT
481: SCGGFEAVAA RGYIYVNITN TGRLDSDYTL TVSNCSSNVR PIEARTLAVR AGSAASLDPP
541: MELYVEDQAA AAARTCTVSL YDSVGAVTDS LTLSFYTNAT QLVVKPSGGY NGTGDGAGVK
601: RNGTDCSTAC TNPIDVLCFV TKKCWSKFGR LLGIIGGALV GLGLLAVALK FGWLASLAAS
661: CCGGGGGAAA GGAGGGMGLG TGGGGGCFGG GQQQQQLPPA ASHAMSPPQQ QQRSHAEVAA
721: GAAVAGAGAA GAAAAVLGAK HGGGGGGARG KQQHADTRHL QDRDSRAIDG GASIGSSSAG
781: GSSSLSSYSQ PREAGGRLLQ PPAAAVFVPE GGGGGAAGDE GARAQSSDWD ARGRSPRVAD
841: EHGSPRQRYD GVRQSPYMVS ANPYDGWYDG GSGGGGGGGG GGYGREAPPP QGPPPHPVGA
901: PPPPPRRRSL WERMWLQRPG GGGGGGGGGG GGGGGGSGGG VDQHGGRSCA DAARRGGGGP
961: GGMRGVEGLM SNGGRPNGPH PHAPPPPPPP QQQQQQQRQR RSFLESLTAM MTLPWGGGRE
1021: EEAGGDRRGG GRGGAAAAHG GRGAGGGRGH PPSIGSPPPG PLQPPEYGPQ GGQARRWGAG
1081: GGRGGVGGDG GGGGVGAAAY VQLSTGGRGG GGGGGRGRGG GREGPTWHNP VYDWQAPPK