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2.A.1.14.30
Vesicular glutamate transporter 1, VGluT1 or PNP1 of 560 aas and 12 TMSs. Brain-specific Na+-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 7). Several proteins must be retrieved to the synaptic vesicle before it can export neurotransmitters, and cargo retrieval is a collective cargo-driven process, dependent on VGluT1 (Pan et al. 2015).  The amino-terminal and carboxyl-terminal regions of VGLUT2 in membranes face the cytoplasm (Jung et al. 2006). It is involved in nervous system diseases (Du et al. 2020). VGLUT1 and VGLUT2, selectively label and define functionally distinct neuronal subpopulations at each relay level of the neural hierarchies comprising spinal and trigeminal sensory systems (Zhang et al. 2018). An overview of the physiologic sites for VGLUT regulation that can modulate glutamate release in an over-active synapse or in a disease state has been presented (Pietrancosta et al. 2020).

Accession Number:Q9P2U7
Protein Name:Vesicular glutamate transporter 1
Length:560
Molecular Weight:61613.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cytoplasmic vesicle1
Substrate glutamate, phosphate, Na+

Cross database links:

Genevestigator: Q9P2U7
eggNOG: COG0477
HEGENOM: HOG000230812
Entrez Gene ID: 57030   
Pfam: PF07690   
KEGG: hsa:57030   

Gene Ontology

GO:0030054 C:cell junction
GO:0060203 C:clathrin sculpted glutamate transport vesicle membrane
GO:0060076 C:excitatory synapse
GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0048786 C:presynaptic active zone
GO:0030672 C:synaptic vesicle membrane
GO:0019717 C:synaptosome
GO:0005315 F:inorganic phosphate transmembrane transporter activity
GO:0005313 F:L-glutamate transmembrane transporter activity
GO:0015321 F:sodium-dependent phosphate transmembrane transporter activity
GO:0015319 F:sodium:inorganic phosphate symporter activity
GO:0014047 P:glutamate secretion
GO:0051938 P:L-glutamate import
GO:0007616 P:long-term memory
GO:0007269 P:neurotransmitter secretion
GO:0060079 P:regulation of excitatory postsynaptic membrane potential
GO:0042137 P:sequestering of neurotransmitter
GO:0035249 P:synaptic transmission, glutamatergic

References (2)

[1] “Molecular cloning of a novel brain-type Na(+)-dependent inorganic phosphate cotransporter.”  Aihara Y.et.al.   10820226
[2] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334

External Searches:

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  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
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  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MEFRQEEFRK LAGRALGKLH RLLEKRQEGA ETLELSADGR PVTTQTRDPP VVDCTCFGLP 
61:	RRYIIAIMSG LGFCISFGIR CNLGVAIVSM VNNSTTHRGG HVVVQKAQFS WDPETVGLIH 
121:	GSFFWGYIVT QIPGGFICQK FAANRVFGFA IVATSTLNML IPSAARVHYG CVIFVRILQG 
181:	LVEGVTYPAC HGIWSKWAPP LERSRLATTA FCGSYAGAVV AMPLAGVLVQ YSGWSSVFYV 
241:	YGSFGIFWYL FWLLVSYESP ALHPSISEEE RKYIEDAIGE SAKLMNPLTK FSTPWRRFFT 
301:	SMPVYAIIVA NFCRSWTFYL LLISQPAYFE EVFGFEISKV GLVSALPHLV MTIIVPIGGQ 
361:	IADFLRSRRI MSTTNVRKLM NCGGFGMEAT LLLVVGYSHS KGVAISFLVL AVGFSGFAIS 
421:	GFNVNHLDIA PRYASILMGI SNGVGTLSGM VCPIIVGAMT KHKTREEWQY VFLIASLVHY 
481:	GGVIFYGVFA SGEKQPWAEP EEMSEEKCGF VGHDQLAGSD DSEMEDEAEP PGAPPAPPPS 
541:	YGATHSTFQP PRPPPPVRDY