2.A.1.14.33
L-galactonate transporter, YjjL or DgoT of 460 aas and 12 TMSs. The D-galactonate transporter DgoT has been solved at atomic resolution (Dmitrieva et al. 2024). Protonation of D46 and E133 precedes galactonate binding, and substrate binding induces closure of the extracellular gate, with the conserved R47 coupling substrate binding to transmembrane helix movement. After isomerization to an inward-facing conformation, deprotonation of E133 and subsequent proton transfer from D46 to E133 opens the intracellular gate and permits galactonate dissociation either in its unprotonated form or after proton transfer from E133. After release of the second proton, apo DgoT returns to the outward-facing conformation (Dmitrieva et al. 2024).