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2.A.1.19.29
Solute carrier family 22 member 1 (Organic cation transporter 1) (hOCT1).  May be a primary polyamine uptake porter (Abdulhussein and Wallace 2013). Amino acids in TMS1 confer major functional differences between human and mouse orthologs of the polyspecific membrane transporter, OCT1. Reduced function alleles of OCT1 associate significantly with high LDL cholesterol levels (Yee et al. 2023).  Structures provided atomic-level insight into the dynamic metformin transfer process via hOCT1 and the mechanism by which spironolactone inhibits it. A 'YER' motif critical for the conformational flexibility of hOCT1 and likely other SLC22 family transporters has been identified (Zhang et al. 2024).  This system is inhibited by various canabis products (Anderson et al. 2022).

Accession Number:O15245
Protein Name:Solute carrier family 22 member 1
Length:554
Molecular Weight:61154.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Basolateral cell membrane1 / Multi-pass membrane protein2
Substrate amino acid, polyamine

Cross database links:

Entrez Gene ID: 6580   
Pfam: PF00083   
KEGG: hsa:6580   

Gene Ontology

GO:0016323 C:basolateral plasma membrane
GO:0005887 C:integral to plasma membrane
GO:0005624 C:membrane fraction
GO:0005277 F:acetylcholine transmembrane transporter activity
GO:0005329 F:dopamine transmembrane transporter activity
GO:0005333 F:norepinephrine transmembrane transporter activity
GO:0015101 F:organic cation transmembrane transporter activity
GO:0015651 F:quaternary ammonium group transmembrane transporter activity
GO:0008513 F:secondary active organic cation transmembrane transporter activity
GO:0006855 P:drug transmembrane transport
GO:0048241 P:epinephrine transport
GO:0010248 P:establishment or maintenance of transmembrane electrochemical gradient

References (18)

[1] “Cloning and characterization of two human polyspecific organic cation transporters.”  Gorboulev V.et.al.   9260930
[2] “Cloning and functional expression of a human liver organic cation transporter.”  Zhang L.et.al.   9187257
[3] “Molecular cloning, functional characterization and genomic organization of four alternatively spliced isoforms of the human organic cation transporter 1 (hOCT1/SLC22A1).”  Hayer M.et.al.   11388889
[4] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[5] “The DNA sequence and analysis of human chromosome 6.”  Mungall A.J.et.al.   14574404
[6] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[7] “Functional characterization of an organic cation transporter (hOCT1) in a transiently transfected human cell line (HeLa).”  Zhang L.et.al.   9655880
[8] “Comparison of 'type I' and 'type II' organic cation transport by organic cation transporters and organic anion-transporting polypeptides.”  van Montfoort J.E.et.al.   11408531
[9] “Regulation of the human organic cation transporter hOCT1.”  Ciarimboli G.et.al.   15389554
[10] “Drug specificity and intestinal membrane localization of human organic cation transporters (OCT).”  Mueller J.et.al.   16263091
[11] “Metformin is a superior substrate for renal organic cation transporter OCT2 rather than hepatic OCT1.”  Kimura N.et.al.   16272756
[12] “The human organic cation transporter-1 gene is transactivated by hepatocyte nuclear factor-4alpha.”  Saborowski M.et.al.   16436500
[13] “Differential pharmacological in vitro properties of organic cation transporters and regional distribution in rat brain.”  Amphoux A.et.al.   16581093
[14] “The expression of the solute carriers NTCP and OCT-1 is regulated by cholesterol in HepG2 cells.”  Dias V.et.al.   17635184
[15] “Identification of genetic variations of the human organic cation transporter hOCT1 and their functional consequences.”  Kerb R.et.al.   12439218
[16] “Evolutionary conservation predicts function of variants of the human organic cation transporter, OCT1.”  Shu Y.et.al.   12719534
[17] “Novel single nucleotide polymorphisms of organic cation transporter 1 (SLC22A1) affecting transport functions.”  Sakata T.et.al.   14697261
[18] “Seven novel single nucleotide polymorphisms in the human SLC22A1 gene encoding organic cation transporter 1 (OCT1).”  Itoda M.et.al.   15499200

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MPTVDDILEQ VGESGWFQKQ AFLILCLLSA AFAPICVGIV FLGFTPDHHC QSPGVAELSQ 
61:	RCGWSPAEEL NYTVPGLGPA GEAFLGQCRR YEVDWNQSAL SCVDPLASLA TNRSHLPLGP 
121:	CQDGWVYDTP GSSIVTEFNL VCADSWKLDL FQSCLNAGFL FGSLGVGYFA DRFGRKLCLL 
181:	GTVLVNAVSG VLMAFSPNYM SMLLFRLLQG LVSKGNWMAG YTLITEFVGS GSRRTVAIMY 
241:	QMAFTVGLVA LTGLAYALPH WRWLQLAVSL PTFLFLLYYW CVPESPRWLL SQKRNTEAIK 
301:	IMDHIAQKNG KLPPADLKML SLEEDVTEKL SPSFADLFRT PRLRKRTFIL MYLWFTDSVL 
361:	YQGLILHMGA TSGNLYLDFL YSALVEIPGA FIALITIDRV GRIYPMAMSN LLAGAACLVM 
421:	IFISPDLHWL NIIIMCVGRM GITIAIQMIC LVNAELYPTF VRNLGVMVCS SLCDIGGIIT 
481:	PFIVFRLREV WQALPLILFA VLGLLAAGVT LLLPETKGVA LPETMKDAEN LGRKAKPKEN 
541:	TIYLKVQTSE PSGT