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2.A.1.2.68
Tetracycline resistance protein, class B (TetA(B)) (Metal-tetracycline/H+ antiporter).  Mutants defective in either transport or tetracycline binding have been isolated (Wright and Tate 2015). Several amino acid substitutions (i.e., D190C, E192C and S201C) alter the specificity of the porter so that it prefers deoxycycline (3x) and minochcline (6x) over tetracycline (Sapunaric and Levy 2005).

Accession Number:P02980
Protein Name:Tetracycline resistance protein, class B
Length:401
Molecular Weight:43267.00
Species:Escherichia coli [562]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate tetracycline

Cross database links:

DIP: DIP-16933N
Entrez Gene ID: 4924775   
Pfam: PF07690   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015297 F:antiporter activity
GO:0046677 P:response to antibiotic

References (5)

[1] “Sequence homology between the tetracycline-resistance determinants of Tn10 and pBR322.”  Nguyen T.T.et.al.   6319234
[2] “Nucleotide sequence of the Tn10 encoded tetracycline resistance gene.”  Hillen W.et.al.   6298728
[3] “Metal-tetracycline/H+ antiporter of Escherichia coli encoded by a transposon Tn10. Histidine 257 plays an essential role in H+ translocation.”  Yamaguchi A.et.al.   1848846
[4] “Metal-tetracycline/H+ antiporter of Escherichia coli encoded by a transposon, Tn10. The role of the conserved dipeptide, Ser65-Asp66, in tetracycline transport.”  Yamaguchi A.et.al.   2168416
[5] “Membrane topology of the transposon 10-encoded metal-tetracycline/H+ antiporter as studied by site-directed chemical labeling.”  Kimura T.et.al.   8995300

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNSSTKIALV ITLLDAMGIG LIMPVLPTLL REFIASEDIA NHFGVLLALY ALMQVIFAPW 
61:	LGKMSDRFGR RPVLLLSLIG ASLDYLLLAF SSALWMLYLG RLLSGITGAT GAVAASVIAD 
121:	TTSASQRVKW FGWLGASFGL GLIAGPIIGG FAGEISPHSP FFIAALLNIV TFLVVMFWFR 
181:	ETKNTRDNTD TEVGVETQSN SVYITLFKTM PILLIIYFSA QLIGQIPATV WVLFTENRFG 
241:	WNSMMVGFSL AGLGLLHSVF QAFVAGRIAT KWGEKTAVLL GFIADSSAFA FLAFISEGWL 
301:	VFPVLILLAG GGIALPALQG VMSIQTKSHQ QGALQGLLVS LTNATGVIGP LLFAVIYNHS 
361:	LPIWDGWIWI IGLAFYCIII LLSMTFMLTP QAQGSKQETS A