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2.A.1.3.4
(Mono- and divalent organocation):H+ antiporter. Transmembrane helix 12 of QacA lines the bivalent cationic drug binding pocket (Hassan et al., 2007). Two sites, D34 and D411 are involved in substrate recognition, while E407 facilitates substrate efflux as a protonation site and plays a role as a substrate recognition site for the transport of dequalinium, a divalent quaternary ammonium compound (Majumder et al. 2019).

Accession Number:P0A0J9
Protein Name:Antiseptic resistance protein QacA aka SAVP032
Length:514
Molecular Weight:55016.00
Species:Staphylococcus aureus [1280]
Number of TMSs:14
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate

Cross database links:

RefSeq: YP_536864.1   
Entrez Gene ID: 3978608   
Pfam: PF07690   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity
GO:0046677 P:response to antibiotic
GO:0055085 P:transmembrane transport

References (2)

[1] “Efflux-mediated antiseptic resistance gene qacA from Staphylococcus aureus: common ancestry with tetracycline- and sugar-transport proteins.”  Rouch D.A.et.al.   2089219
[2] “Multidrug resistance proteins QacA and QacB from Staphylococcus aureus: membrane topology and identification of residues involved in substrate specificity.”  Paulsen I.T.et.al.   8622987

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MISFFTKTTD MMTSKKRWTA LVVLAVSLFV VTMDMTILIM ALPELVRELE PSGTQQLWIV 
61:	DIYSLVLAGF IIPLSAFADK WGRKKALLTG FALFGLVSLA IFFAESAEFV IAIRFLLGIA 
121:	GALIMPTTLS MIRVIFENPK ERATALAVWS IASSIGAVFG PIIGGALLEQ FSWHSAFLIN 
181:	VPFAIIAVVA GLFLLPESKL SKEKSHSWDI PSTILSIAGM IGLVWSIKEF SKEGLADIIP 
241:	WVVIVLAITM IVIFVKRNLS SSDPMLDVRL FKKRSFSAGT IAAFMTMFAM ASVLLLASQW 
301:	LQVVEELSPF KAGLYLLPMA IGDMVFAPIA PGLAARFGPK IVLPSGIGIA AIGMFIMYFF 
361:	GHPLSYSTMA LALILVGAGM ASLAVASALI MLETPTSKAG NAAAVEESMY DLGNVFGVAV 
421:	LGSLSSMLYR VFLDISSFSS KGIVGDLAHV AEESVVGAVE VAKATGIKQL ANEAVTSFND 
481:	AFVATALVGG IIMIIISIVV YLLIPKSLDI TKQK