Search TCDB: (?)
TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.23.2.8
Excitatory amino acid transporter 4, EAAT4 (Sodium-dependent glutamate/aspartate transporter) (Solute carrier family 1 member 6).  Klotho, a 1012 aa protein with N- and C-terminal TMSs, is a regulator of the excitatory amino acid transporters EAAT3 and EAAT4 (Almilaji et al. 2013).  Phorbol 12-myristate 13-acetate (PMA), a protein kinase C (PKC) activator, enhanced Cl- currents via EAAT4, but this increased Cl- current was not thermodynamically coupled to glutamate transport. These PMA-enhanced Cl- currents were partially blocked by staurosporine, chelerythrine, and calphostin C, the three PKC inhibitors, implying that PKC-mediated phsophorylation was responsible (Fang et al. 2006).

Accession Number:P48664
Protein Name:Excitatory amino acid transporter 4
Length:564
Molecular Weight:61565.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate sodium(1+), glutamate(2-), aspartate(2-)

Cross database links:

Entrez Gene ID: 6511   
Pfam: PF00375   
KEGG: hsa:6511   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0005624 C:membrane fraction
GO:0015183 F:L-aspartate transmembrane transporter activity
GO:0005313 F:L-glutamate transmembrane transporter activity
GO:0017153 F:sodium:dicarboxylate symporter activity
GO:0015813 P:L-glutamate transport
GO:0042391 P:regulation of membrane potential
GO:0007268 P:synaptic transmission

References (2)

[1] “An excitatory amino-acid transporter with properties of a ligand-gated chloride channel.”  Fairman W.A.et.al.   7791878
[2] “The DNA sequence and biology of human chromosome 19.”  Grimwood J.et.al.   15057824

External Searches:

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence 
1:	MSSHGNSLFL RESGQRLGRV GWLQRLQESL QQRALRTRLR LQTMTLEHVL RFLRRNAFIL 
61:	LTVSAVVIGV SLAFALRPYQ LTYRQIKYFS FPGELLMRML QMLVLPLIVS SLVTGMASLD 
121:	NKATGRMGMR AAVYYMVTTI IAVFIGILMV TIIHPGKGSK EGLHREGRIE TIPTADAFMD 
181:	LIRNMFPPNL VEACFKQFKT QYSTRVVTRT MVRTENGSEP GASMPPPFSV ENGTSFLENV 
241:	TRALGTLQEM LSFEETVPVP GSANGINALG LVVFSVAFGL VIGGMKHKGR VLRDFFDSLN 
301:	EAIMRLVGII IWYAPVGILF LIAGKILEME DMAVLGGQLG MYTLTVIVGL FLHAGIVLPL 
361:	IYFLVTHRNP FPFIGGMLQA LITAMGTSSS SATLPITFRC LEEGLGVDRR ITRFVLPVGA 
421:	TVNMDGTALY EALAAIFIAQ VNNYELNLGQ ITTISITATA ASVGAAGIPQ AGLVTMVIVL 
481:	TSVGLPTEDI TLIIAVDWFL DRLRTMTNVL GDSIGAAVIE HLSQRELELQ EAELTLPSLG 
541:	KPYKSLMAQE KGASRGRGGN ESAM