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2.A.23.2.8
Excitatory amino acid transporter 4, EAAT4 (Sodium-dependent glutamate/aspartate transporter) (Solute carrier family 1 member 6).  Klotho, a 1012 aa protein with N- and C-terminal TMSs, is a regulator of the excitatory amino acid transporters EAAT3 and EAAT4 (Almilaji et al. 2013).  Phorbol 12-myristate 13-acetate (PMA), a protein kinase C (PKC) activator, enhanced Cl- currents via EAAT4, but this increased Cl- current was not thermodynamically coupled to glutamate transport. These PMA-enhanced Cl- currents were partially blocked by staurosporine, chelerythrine, and calphostin C, the three PKC inhibitors, implying that PKC-mediated phsophorylation was responsible (Fang et al. 2006). Epispdic ataxia (EA6) is caused by mutations in SLC1A3 encoding this glutamate transporter that is also an anion channel (Graves et al. 2024).

Accession Number:P48664
Protein Name:Excitatory amino acid transporter 4
Length:564
Molecular Weight:61565.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate sodium(1+), glutamate(2-), aspartate(2-)

Cross database links:

Entrez Gene ID: 6511   
Pfam: PF00375   
KEGG: hsa:6511   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0005624 C:membrane fraction
GO:0015183 F:L-aspartate transmembrane transporter activity
GO:0005313 F:L-glutamate transmembrane transporter activity
GO:0017153 F:sodium:dicarboxylate symporter activity
GO:0015813 P:L-glutamate transport
GO:0042391 P:regulation of membrane potential
GO:0007268 P:synaptic transmission

References (2)

[1] “An excitatory amino-acid transporter with properties of a ligand-gated chloride channel.”  Fairman W.A.et.al.   7791878
[2] “The DNA sequence and biology of human chromosome 19.”  Grimwood J.et.al.   15057824

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MSSHGNSLFL RESGQRLGRV GWLQRLQESL QQRALRTRLR LQTMTLEHVL RFLRRNAFIL 
61:	LTVSAVVIGV SLAFALRPYQ LTYRQIKYFS FPGELLMRML QMLVLPLIVS SLVTGMASLD 
121:	NKATGRMGMR AAVYYMVTTI IAVFIGILMV TIIHPGKGSK EGLHREGRIE TIPTADAFMD 
181:	LIRNMFPPNL VEACFKQFKT QYSTRVVTRT MVRTENGSEP GASMPPPFSV ENGTSFLENV 
241:	TRALGTLQEM LSFEETVPVP GSANGINALG LVVFSVAFGL VIGGMKHKGR VLRDFFDSLN 
301:	EAIMRLVGII IWYAPVGILF LIAGKILEME DMAVLGGQLG MYTLTVIVGL FLHAGIVLPL 
361:	IYFLVTHRNP FPFIGGMLQA LITAMGTSSS SATLPITFRC LEEGLGVDRR ITRFVLPVGA 
421:	TVNMDGTALY EALAAIFIAQ VNNYELNLGQ ITTISITATA ASVGAAGIPQ AGLVTMVIVL 
481:	TSVGLPTEDI TLIIAVDWFL DRLRTMTNVL GDSIGAAVIE HLSQRELELQ EAELTLPSLG 
541:	KPYKSLMAQE KGASRGRGGN ESAM