2.A.26.1.10
Branched chain amino acid transporter 2 of 439 aas and 12 TMSs, BrnQ (Guardiola et al. 1974). The brnQ gene can be mutated to give rise to threonine tolerance (Radi et al. 2022). However the wild type protein takes up threonine (in addition to leucine, isoleucine and valine) but with low affinity and high flux (Khozov et al. 2023).
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| Accession Number: | P0AD99 |
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| Protein Name: | Branched-chain amino acid transport system 2 carrier protein |
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| Length: | 439 |
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| Molecular Weight: | 46209.00 |
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| Species: | Escherichia coli (strain K12) [83333] |
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| Number of TMSs: | 12 |
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| Location1 / Topology2 / Orientation3: |
Cell inner membrane1 / Multi-pass membrane protein2 |
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| Substrate |
L-threonine, L-isoleucine, branched-chain amino acid, L-valine, L-leucine |
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1: MTHQLRSRDI IALGFMTFAL FVGAGNIIFP PMVGLQAGEH VWTAAFGFLI TAVGLPVLTV
61: VALAKVGGGV DSLSTPIGKV AGVLLATVCY LAVGPLFATP RTATVSFEVG IAPLTGDSAL
121: PLFIYSLVYF AIVILVSLYP GKLLDTVGNF LAPLKIIALV ILSVAAIVWP AGSISTATEA
181: YQNAAFSNGF VNGYLTMDTL GAMVFGIVIV NAARSRGVTE ARLLTRYTVW AGLMAGVGLT
241: LLYLALFRLG SDSASLVDQS ANGAAILHAY VQHTFGGGGS FLLAALIFIA CLVTAVGLTC
301: ACAEFFAQYV PLSYRTLVFI LGGFSMVVSN LGLSQLIQIS VPVLTAIYPP CIALVVLSFT
361: RSWWHNSSRV IAPPMFISLL FGILDGIKAS AFSDILPSWA QRLPLAEQGL AWLMPTVVMV
421: VLAIIWDRAA GRQVTSSAH