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2.A.3.10.10
Lysine permease of 611 aas and 13 putative TMSs, Lyp1.  Extracellular loops affect either the localization or activity of Lyp1. Half of the mutants are located in the extension of extracellular loop 3 or in a predicted alpha-helix in extracellular loop 4 (Van't Klooster et al. 2020). Phosphatidylserine and ergosterol are essential for Lyp1 function, and the transport activity displays a sigmoidal relationship with the concentration of these lipids. Lyp1 requires a relatively high fraction of lipids with one or more unsaturated acyl chains. Possibly a narrow band of lipids immediately surrounding the transmembrane stalk of a model protein allows conformational changes in the protein (Van't Klooster et al. 2020).

Accession Number:P32487
Protein Name:Lyp1 aka YNL268W aka N0790
Length:611
Molecular Weight:68090.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate lysine

Cross database links:

Genevestigator: P32487
eggNOG: fuNOG04851
HEGENOM: HBG492579
RefSeq: NP_014131.1   
Entrez Gene ID: 855453   
Pfam: PF00324   
KEGG: sce:YNL268W   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005739 C:mitochondrion
GO:0015174 F:basic amino acid transmembrane transporter ...
GO:0005515 F:protein binding
GO:0015802 P:basic amino acid transport
GO:0055085 P:transmembrane transport

References (9)

[1] “Cloning and sequencing of the Saccharomyces cerevisiae gene LYP1 coding for a lysine-specific permease.”  Sychrova H.et.al.   8368011
[2] “The sequence of a 24,152 bp segment from the left arm of chromosome XIV from Saccharomyces cerevisiae between the BNI1 and the POL2 genes.”  Sen-Gupta M.et.al.   8740425
[3] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.”  Philippsen P.et.al.   9169873
[4] “Substrate specificity and gene expression of the amino-acid permeases in Saccharomyces cerevisiae.”  Regenberg B.et.al.   10654085
[5] “Global analysis of protein expression in yeast.”  Ghaemmaghami S.et.al.   14562106
[6] “A proteomics approach to understanding protein ubiquitination.”  Peng J.et.al.   12872131
[7] “Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.”  Gruhler A.et.al.   15665377
[8] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
[9] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

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  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MGRFSNIITS NKWDEKQNNI GEQSMQELPE DQIEHEMEAI DPSNKTTPYS IDEKQYNTKK 
61:	KHGSLQGGAI ADVNSITNSL TRLQVVSHET DINEDEEEAH YEDKHVKRAL KQRHIGMIAL 
121:	GGTIGTGLFV GISTPLSNAG PVGSLIAYIF MGTIVYFVTQ SLGEMATFIP VTSSITVFSK 
181:	RFLSPAFGVS NGYMYWFNWA ITYAVEVSVI GQVIEYWTDK VPLAAWIAIF WVIITLMNFF 
241:	PVKVYGEFEF WVASVKVLAI MGYLIYALII VCGGSHQGPI GFRYWRNPGA WGPGIISSDK 
301:	SEGRFLGWVS SLINAAFTYQ GTELVGITAG EAANPRKTVP RAINKVVFRI VLFYIMSLFF 
361:	IGLLVPYNDS RLSASSAVIA SSPFVISIQN AGTYALPDIF NAVVLITVVS AANSNVYVGS 
421:	RVLYSLARTG NAPKQFGYVT RQGVPYLGVV CTAALGLLAF LVVNNNANTA FNWLINISTL 
481:	AGLCAWLFIS LAHIRFMQAL KHRGISRDDL PFKAKLMPYG AYYAAFFVTV IIFIQGFQAF 
541:	CPFKVSEFFT SYISLILLAV VFIGCQIYYK CRFIWKLEDI DIDSDRREIE AIIWEDDEPK 
601:	NLWEKFWAAV A