Search TCDB: (?)
TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.30.1.11
NaCl symporter (activated by phosphorylation of the N-terminal domain upon Cl- depletion (Pacheco-Alvarez et al., 2006)). The thiazide sensitive Na+:Cl- cotransporter (NCC) provides the principal route for salt reabsorption in the apical membrane of the distal convoluted tubule (DCT) in mammals and plays a fundamental role in managing blood pressure. The cotransporter is targeted by thiazide diuretics, a highly prescribed medication that is effective in treating arterial hypertension and edema (Moreno et al. 2023). The structure has been solved by cryoEM, and the structure/function relationships have been reviewed (Moreno et al. 2023).

Accession Number:P55018
Protein Name:TSCC aka SLC12A3 aka TSC
Length:1002
Molecular Weight:110554.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate

Cross database links:

RefSeq: NP_062218.3   
Entrez Gene ID: 54300   
Pfam: PF00324    PF08403   
KEGG: rno:54300   

Gene Ontology

GO:0016324 C:apical plasma membrane
GO:0016021 C:integral to membrane
GO:0031982 C:vesicle
GO:0015378 F:sodium:chloride symporter activity
GO:0006821 P:chloride transport
GO:0006814 P:sodium ion transport
GO:0055085 P:transmembrane transport

References (1)

[1] “Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney.”  Gamba G.et.al.   8021284

External Searches:

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence 
1:	MAELPVTEMP GDALCSGRFT ISTLLGGDEP PPAACDNSQP SHLTHGSTLY LRTFGYNTID 
61:	VVPAYEHYAN SALPGEPRKV RPTLADLHSF LKQEGSHLHA LAFDGRPGHE LTDGLVEDET 
121:	GANSEKSPGE PVRFGWVKGV MIRCMLNIWG VILYLRLPWI TAQAGIVLTW LIILLSVMVT 
181:	SITGLSISAI STNGKVKSGG TYFLISRSLG PELGGSIGLI FAFANAVGVA MHTVGFAETV 
241:	RDLLQENGTP IVDPINDIRI IGVVTVTVLL AISLAGMEWE SKAQVLFFLV IMVSFANYLV 
301:	GTLIPASKDK ASKGFYSYHG DIFVQNLVPD WRGIDGSFFG MFSIFFPSAT GILAGANISG 
361:	DLKDPAVAIP KGTLMAIFWT TISYLAISAT IGSCVVRDAS GDVNDTITPG PGLCEGLACG 
421:	YGWNFTECSQ QHSCRYGLIN YYQTMSMVSA FAPLITAGIF GATLSSALAC LVSAAKVFQC 
481:	LCEDQLYPLI GFFGKGYGKN KEPVRGYLLA YAIAVAFIII AELNTIAPII SNFFLCSYAL 
541:	INFSCFHASI TNSPGWRPSF RYYSKWAALF GAVISVVIMF LLTWWAALIA IGVVLFLLLY 
601:	VIYKKPEVNW GSSVQAGSYN LALSYSVGLN EVEDHIKNYR PQCLVLTGPP NFRPALVDFV 
661:	STFTQNLSLM ICGHVLIAPG KQRVPELRLI ASGHTKWLNK RKIKAFYSDV IAEDLRSGVQ 
721:	ILMQASGLGR MKPNILVVGF KKNWQSAHPA TLEDYIGILH DAFDFNYGVC IMRMREGLNV 
781:	SEALQTHTAP EALVQEEQTS TIFQSEQGKK TIDIYWLFDD GGLTLLIPYL LHRKKRWGKC 
841:	KIRVFVGGQI NRMDEERKAI ISLLSKFRLG FHEVHVLPDI NQKPQVEHTK RFEDMIAPFR 
901:	LNDGFKDEAT VAEMRRDYPW KISDEEINKN RIKSLRQVRL NEILLDYSRD AALIILTLPI 
961:	GRKGKCPSSL YMAWLETLSQ DLSPPVLLIR GNQENVLTFY CQ