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2.A.31.2.8
Electrogenic HCO3-:Na+ symporter, NBCe2 = NBC4 or SLC4A5 (3:1 stoichiometry) (Millar and Brown, 2008). Electrogenic sodium bicarbonate cotransporter 4, NBC4 or SLC4A5, of 1137 aas and probably 12 TMSs in a 4 + 4 + 2 + 2 TMS arrangement (Sassani et al. 2002). NBC1 and NBC4 are electrogenic due to the coupling of one anionic and two cationic fluxes during each transport cycle. Most other sodium bicarbonate cotransporters and members of the bicarbonate transporter superfamily catalyze electroneutral processes (Kurtz et al. 2004). It may have a housekeeping function in regulating the pH of tissues in which it is expressed. It may also play a role in mediating Na+:HCO3- cotransport in hepatocytes and intrahepatic cholangiocytes and could be important in protecting the renal paranchyma from alterations in the urinary pH.

Accession Number:Q9BY07
Protein Name:NBCe2
Length:1137
Molecular Weight:126255.00
Species:Mus Musculus (Mouse) [9606]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Apical cell membrane1 / Multi-pass membrane protein2
Substrate sodium(1+), hydrogencarbonate

Cross database links:

RefSeq: NP_067019.3    NP_597812.1   
Entrez Gene ID: 57835   
Pfam: PF07565    PF00955   
OMIM: 606757  gene
KEGG: hsa:57835    hsa:57835   

Gene Ontology

GO:0016324 C:apical plasma membrane
GO:0016021 C:integral to membrane
GO:0005452 F:inorganic anion exchanger activity
GO:0008510 F:sodium:bicarbonate symporter activity
GO:0015701 P:bicarbonate transport
GO:0006814 P:sodium ion transport
GO:0005886 C:plasma membrane

References (16)

[1] “Cloning, characterization and chromosomal assignment of NBC4, a new member of the sodium bicarbonate cotransporter family.”  Pushkin A.et.al.   10978526
[2] “Two C-terminal variants of NBC4, a new member of the sodium bicarbonate cotransporter family: cloning, characterization, and localization.”  Pushkin A.et.al.   11087115
[3] “Genomic organization of the DCTN1-SLC4A5 locus encoding both NBC4 and p150(Glued).”  Pushkin A.et.al.   12063394
[4] “Functional characterization of NBC4: a new electrogenic sodium-bicarbonate cotransporter.”  Sassani P.et.al.   11788353
[5] “Functional characterization of human NBC4 as an electrogenic Na+-HCO cotransporter (NBCe2).”  Virkki L.V.et.al.   11997242
[6] “Expression of the Na+-HCO-3 cotransporter NBC4 in rat kidney and characterization of a novel NBC4 variant.”  Xu J.et.al.   12388414
[7] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[8] “Molecular expression of SLC4-derived Na+-dependent anion transporters in selected human tissues.”  Damkier H.H.et.al.   17715183
[9] “Cloning, characterization and chromosomal assignment of NBC4, a new member of the sodium bicarbonate cotransporter family.”  Pushkin A.et.al.   10978526
[10] “Two C-terminal variants of NBC4, a new member of the sodium bicarbonate cotransporter family: cloning, characterization, and localization.”  Pushkin A.et.al.   11087115
[11] “Genomic organization of the DCTN1-SLC4A5 locus encoding both NBC4 and p150(Glued).”  Pushkin A.et.al.   12063394
[12] “Functional characterization of NBC4: a new electrogenic sodium-bicarbonate cotransporter.”  Sassani P.et.al.   11788353
[13] “Functional characterization of human NBC4 as an electrogenic Na+-HCO cotransporter (NBCe2).”  Virkki L.V.et.al.   11997242
[14] “Expression of the Na+-HCO-3 cotransporter NBC4 in rat kidney and characterization of a novel NBC4 variant.”  Xu J.et.al.   12388414
[15] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[16] “Molecular expression of SLC4-derived Na+-dependent anion transporters in selected human tissues.”  Damkier H.H.et.al.   17715183

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MKVKEEKAGV GKLDHTNHRR RFPDQKECPP IHIGLPVPTY PQRKTDQKGH LSGLQKVHWG 
61:	LRPDQPQQEL TGPGSGASSQ DSSMDLISRT RSPAAEQLQD ILGEEDEAPN PTLFTEMDTL 
121:	QHDGDQMEWK ESARWIKFEE KVEEGGERWS KPHVSTLSLH SLFELRTCLQ TGTVLLDLDS 
181:	GSLPQIIDDV IEKQIEDGLL RPELRERVSY VLLRRHRHQT KKPIHRSLAD IGKSVSTTNR 
241:	SPARSPGAGP SLHHSTEDLR MRQSANYGRL CHAQSRSMND ISLTPNTDQR KNKFMKKIPK 
301:	DSEASNVLVG EVDFLDQPFI AFVRLIQSAM LGGVTEVPVP TRFLFILLGP SGRAKSYNEI 
361:	GRAIATLMVD DLFSDVAYKA RNREDLIAGI DEFLDEVIVL PPGEWDPNIR IEPPKKVPSA 
421:	DKRKSVFSLA ELGQMNGSVG GGGGAPGGGN GGGGGGGSGG GAGSGGAGGT SSGDDGEMPA 
481:	MHEIGEELIW TGRFFGGLCL DIKRKLPWFP SDFYDGFHIQ SISAILFIYL GCITNAITFG 
541:	GLLGDATDNY QGVMESFLGT AMAGSLFCLF SGQPLIILSS TGPILIFEKL LFDFSKGNGL 
601:	DYMEFRLWIG LHSAVQCLIL VATDASFIIK YITRFTEEGF STLISFIFIY DAIKKMIGAF 
661:	KYYPINMDFK PNFITTYKCE CVAPDTVNTT VFNASAPLAP DTNASLYNLL NLTALDWSLL 
721:	SKKECLSYGG RLLGNSCKFI PDLALMSFIL FFGTYSMTLT LKKFKFSRYF PTKVRALVAD 
781:	FSIVFSILMF CGIDACFGLE TPKLHVPSVI KPTRPDRGWF VAPFGKNPWW VYPASILPAL 
841:	LVTILIFMDQ QITAVIVNRK ENKLKKAAGY HLDLFWVGIL MALCSFMGLP WYVAATVISI 
901:	AHIDSLKMET ETSAPGEQPQ FLGVREQRVT GIIVFILTGI SVFLAPILKC IPLPVLYGVF 
961:	LYMGVASLNG IQMGTGGSEF KIQKKLTPFW ERCKLFLMPA KHQPDHAFLR HVPLRRIHLF 
1021:	TLVQILCLAV LWILKSTVAA IIFPVMILGL IIVRRLLDFI FSQHDLAWID NILPEKEKKE 
1081:	TDKKRKRKKG AHEDCDEEPQ FPPPSVIKIP MESVQSDPQN GIHCIARKRS SSWSYSL