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2.A.36.1.1
Na+:H+ antiporter 1 (Nhe-1) (Regulated by Daxx (O35613)). An integral membrane protein that regulates intracellular pH and has a large N-terminal membrane domain of 12 transmembrane segments and an intracellular C-terminal regulatory domain (Reddy et al., 2008). The dimer catalyzes antiport with 2Na+/2H+ stoichiometry (Fuster et al., 2008). Nedd4-1 and β-arrestin-1 are key regulators of Na+/H+ exchanger 1 ubiquitylation, endocytosis and function (Simonin and Fuster et al., 2010). Important in heart disease and cancer. Structural studies have been performed using NMR (Lee et al., 2011). Analyses of heart disease and the involvement of cell regulatory systems have been proposed (Li et al. 2023).

Accession Number:P26431
Protein Name:NAH1 aka Nhe-1 aka SLC9A1 aka NHE1
Length:820
Molecular Weight:91647.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:13
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate sodium(1+), hydron

Cross database links:

RefSeq: NP_036784.1   
Entrez Gene ID: 24782   
Pfam: PF00999   
KEGG: rno:24782   

Gene Ontology

GO:0016323 C:basolateral plasma membrane
GO:0016021 C:integral to membrane
GO:0045121 C:membrane raft
GO:0015385 F:sodium:hydrogen antiporter activity
GO:0008134 F:transcription factor binding
GO:0030154 P:cell differentiation
GO:0016049 P:cell growth
GO:0045768 P:positive regulation of anti-apoptosis
GO:0007243 P:intracellular protein kinase cascade
GO:0006885 P:regulation of pH
GO:0042493 P:response to drug
GO:0014070 P:response to organic cyclic substance
GO:0006814 P:sodium ion transport
GO:0055085 P:transmembrane transport

References (4)

[1] “Molecular cloning of putative members of the Na/H exchanger gene family. cDNA cloning, deduced amino acid sequence, and mRNA tissue expression of the rat Na/H exchanger NHE-1 and two structurally related proteins.”  Orlowski J.et.al.   1577762
[2] “Topological analysis of NHE1, the ubiquitous Na+/H+ exchanger using chymotryptic cleavage.”  Shrode L.D.et.al.   9688597
[3] “Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS.”  Moser K.et.al.   16396499
[4] “Calcineurin B homologous protein 3 promotes the biosynthetic maturation, cell surface stability, and optimal transport of the Na+/H+ exchanger NHE1 isoform.”  Zaun H.C.et.al.   18321853

External Searches:

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MMLRWSGIWG LYPPRIFPSL LVVVALVGLL PVLRSHGLQL NPTASTIRGS EPPRERSIGD 
61:	VTTAPSEPLH HPDDRNLTNL YIEHGAKPVR KAFPVLDIDY LHVRTPFEIS LWILLACLMK 
121:	IGFHVIPTIS SIVPESCLLI VVGLLVGGLI KGVGETPPFL QSDVFFLFLL PPIILDAGYF 
181:	LPLRQFTENL GTILIFAVVG TLWNAFFLGG LLYAVCLVGG EQINNIGLLD TLLFGSIISA 
241:	VDPVAVLAVF EEIHINELLH ILVFGESLLN DAVTVVLYHL FEEFASYEYV GISDIFLGFL 
301:	SFFVVSLGGV FVGVVYGVIA AFTSRFTSHI RVIEPLFVFL YSYMAYLSAE LFHLSGIMAL 
361:	IASGVVMRPY VEANISHKSH TTIKYFLKMW SSVSETLIFI FLGVSTVAGS HQWNWTFVIS 
421:	TLLFCLIARV LGVLVLTWFI NKFRIVKLTP KDQFIIAYGG LRGAIAFSLG YLLDKKHFPM 
481:	CDLFLTAIIT VIFFTVFVQG MTIRPLVDLL AVKKKQETKR SINEEIHTQF LDHLLTGIED 
541:	ICGHYGHHHW KDKLNRFNKK YVKKCLIAGE RSKEPQLIAF YHKMEMKQAI ELVESGGMGK 
601:	IPSAVSTVSM QNIHPKSAAS ERILPALSKD KEEEIRKILR SNLQKTRQRL RSYNRHTLVA 
661:	DPYEEAWNQM LLRRQKARQL EQKITNYLTV PAHKLDSPTM SRARIGSDPL AYEPKADLPV 
721:	ITIDPASPQS PESVDLVNEE LKGKVLGLKR GPRTTPEEEE EDEDGVIMIR SKEPSSPGTD 
781:	DVFTPGPSDS PGSQRIQRCL SDPGPHPEPG EGEPFIPKGQ