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2.A.38.2.1
High affinity K+ transporter (K+:H+ symporter). Trk1 is functional without its "long hydrophilic loop", but this LHL regulates cation translocation activity and selectivity (Kale et al. 2019). Possibly, LHL influences a transmembrane helix (M2A) which can switch between bent and straight conformations, thereby directly modifying the radius of the pore and selectivity filter.  Distinct regions of its first intracellular loop contribute to the proper localization, transport activity and substrate-affinity adjustment of the main yeast K+ importer, Trk1 (Papouskova et al. 2024).

Accession Number:P12685
Protein Name:Trk1 aka YJL129C aka J0693
Length:1235
Molecular Weight:141073.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:9
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate potassium(1+), hydron

Cross database links:

DIP: DIP-7454N
RefSeq: NP_012406.1   
Entrez Gene ID: 853312   
Pfam: PF02386   
KEGG: sce:YJL129C   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0045121 C:membrane raft
GO:0015079 F:potassium ion transmembrane transporter act...
GO:0030007 P:cellular potassium ion homeostasis
GO:0006813 P:potassium ion transport
GO:0055085 P:transmembrane transport

References (5)

[1] “TRK1 encodes a plasma membrane protein required for high-affinity potassium transport in Saccharomyces cerevisiae.”  Gaber R.F.et.al.   3043197
[2] “Sequencing analysis of a 40.2 kb fragment of yeast chromosome X reveals 19 open reading frames including URA2 (5' end), TRK1, PBS2, SPT10, GCD14, RPE1, PHO86, NCA3, ASF1, CCT7, GZF3, two tRNA genes, three remnant delta elements and a Ty4 transposon.”  Cziepluch C.et.al.   8948101
[3] “Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.”  Galibert F.et.al.   8641269
[4] “Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.”  Li X.et.al.   17330950
[5] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MHFRRTMSRV PTLASLEIRY KKSFGHKFRD FIALCGHYFA PVKKYIFPSF IAVHYFYTIS 
61:	LTLITSILLY PIKNTRYIDT LFLAAGAVTQ GGLNTVDINN LSLYQQIVLY IVCCISTPIA 
121:	VHSCLAFVRL YWFERYFDGI RDSSRRNFKM RRTKTILERE LTARTMTKNR TGTQRTSYPR 
181:	KQAKTDDFQE KLFSGEMVNR DEQDSVHSDQ NSHDISRDSS NNNTNHNGSS GSLDDFVKED 
241:	ETDDNGEYQE NNSYSTVGSS SNTVADESLN QKPKPSSLRF DEPHSKQRPA RVPSEKFAKR 
301:	RGSRDISPAD MYRSIMMLQG KHEATAEDEG PPLVIGSPAD GTRYKSNVNK LKKATGINGN 
361:	KIKIRDKGNE SNTDQNSVSS EANSTASVSD ESSLHTNFGN KVPSLRTNTH RSNSGPIAIT 
421:	DNAETDKKHG PSIQFDITKP PRKISKRVST FDDLNPKSSV LYRKKASKKY LMKHFPKARR 
481:	IRQQIKRRLS TGSIEKNSSN NVSDRKPITD MDDDDDDDDN DGDNNEEYFA DNESGDEDER 
541:	VQQSEPHSDS ELKSHQQQQE KHQLQQNLHR MYKTKSFDDN RSRAVPMERS RTIDMAEAKD 
601:	LNELARTPDF QKMVYQNWKA HHRKKPNFRK RGWNNKIFEH GPYASDSDRN YPDNSNTGNS 
661:	ILHYAESILH HDGSHKNGSE EASSDSNENI YSTNGGSDHN GLNNYPTYND DEEGYYGLHF 
721:	DTDYDLDPRH DLSKGSGKTY LSWQPTIGRN SNFLGLTRAQ KDELGGVEYR AIKLLCTILV 
781:	VYYVGWHIVA FVMLVPWIIL KKHYSEVVRD DGVSPTWWGF WTAMSAFNDL GLTLTPNSMM 
841:	SFNKAVYPLI VMIWFIIIGN TGFPILLRCI IWIMFKISPD LSQMRESLGF LLDHPRRCFT 
901:	LLFPKAATWW LLLTLAGLNI TDWILFIILD FGSTVVKSLS KGYRVLVGLF QSVSTRTAGF 
961:	SVVDLSQLHP SIQVSYMLMM YVSVLPLAIS IRRTNVYEEQ SLGLYGDMGG EPEDTDTEDD 
1021:	GNDEDDDEEN ESHEGQSSQR SSSNNNNNNN RKKKKKKKTE NPNEISTKSF IGAHLRKQLS 
1081:	FDLWFLFLGL FIICICEGDK IKDVQEPNFN IFAILFEIVS AYGTVGLSLG YPDTNQSFSR 
1141:	QFTTLSKLVI IAMLIRGKNR GLPYSLDRAI ILPSDRLEHI DHLEGMKLKR QARTNTEDPM 
1201:	TEHFKRSFTD VKHRWGALKR KTTHSRNPKR SSTTL