2.A.45.2.7
Citrate transporter, OsCT1 of 567 aas and 12 TMSs in a 3 + 1 + 1 + 1 + 3 + 1 + 1 + 1 TMS arrangement. It differs from previously known plant citrate
transporters and is structurally close to rice silicon transporters (Panchal et al. 2023). OsCT1 carries a bacterial citrate-metal transporter domain, CitMHS.
and showed citrate efflux activity when expressed in Xenopus laevis oocytes, being localized to the cell plasma membrane. It is highly
expressed in the shoots and reproductive tissues of rice, and its
promoter activity was visible in cells surrounding the vasculature. The
OsCT1 knockout (KO) lines showed a reduced citrate content in the shoots
and the root exudates, whereas the overexpression (OE) line showed higher
citrate exudation from their roots. Further, the KO and OE lines showed
variations in the manganese (Mn) distribution as expected if citrate and Mn2+ were co-transported.
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| Accession Number: | XP_015614851.1 |
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| Protein Name: | XP_015614851.1 silicon efflux transporter LSI2-like [Oryza sativa Japonica Group] |
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| Length: | 568 |
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| Molecular Weight: | |
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| Species: | Oryza sativa Japonica Group [39947] |
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| Number of TMSs: | 11 |
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| Substrate |
citrate salt, manganese(2+) |
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1: MVLASTPKVV LGCVAFTIFW VLAVFPSVPF MPVGRTAGSL LGAMLMVLFR VMTPEEAYAA
61: IDLPILGLLF GTMVVSIFLE RADMFKYLGN MLSWKSRGSK DLLFRVCVVS AVASALFTND
121: TTCVVLTEFI LKVARQNNLP PQPFLLALAS SSNIGSAATP IGNPQNLVIA VESGITFGQF
181: LLGVFPAMVV GILANTCILL CYFWRYLSAD RDQLEGGGGA HGPEVVADEE VTSHRFTPAR
241: MSHASSVNDS DCISEPIRRS ESMNRADALR SRSYNSEGDI QVAIRSLRAS SLSREMVEVS
301: TVCDRRDVVV DGGGGGGPRK ITRSTSHQRS VIIEDAPEQH QHPAGLFDGE KDKDDEAIGK
361: RRRWKVIVWK YAVYLTTLGM LAALLLGLNM SWTAITAALI LLALDFTDAQ ACLEKVSYSL
421: LIFFCGMFIT VDGFNKTGIP NTLWELVEPY ARIDSPKGVV LLAIVILVLS NVASNVPTVL
481: LLGTRVAASA AAISPASEKK AWLILAWVST VAGNLTLLGS AANLIVCEQA RRAQFFGYNL
541: TFWSHLRFGV PSTIIVTAIG LLIVMSY