2.A.55.2.23
Natural resistance-associated macrophage protein, MsNRAMP of 554 aas and 12 TMSs. Chesapeake Bay striped bass (Morone saxatilis) is experiencing an epizootic of mycobacteriosis that threatens the health of this species. Burge et al. 2004 characterized an Nramp gene in this species and show that induction follows Mycobacterium exposure. MsNramp contains all the signal features of the Nramp family, including a topology of 12 transmembrane domains (TM), the transport protein-specific binding-protein-dependent transport system inner membrane component signature, three N-linked glycosylation sites between TMSs 7 and 8, sites of casein kinase and protein kinase C phosphorylation in the amino and carboxy termini, and a tyrosine kinase phosphorylation site between TMSs 6 and 7. MsNramp was present in all tissues assayed. Within 1 day of injection of Mycobacterium marinum, MsNramp expression was highly induced (17-fold higher) in peritoneal exudate (PE) cells (Burge et al. 2004).