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2.A.56.1.3
Na+-dependent (smf-driven) sialic acid (N-acetyl neuraminic acid) transporter, SiaTP (Allen et al., 2005; Severi et al., 2005; Johnston et al., 2008). SiaT is also called SiaQM (Mulligan et al., 2009).  Also transports the related sialic acids, N-glycolylneuraminic acid (Neu5Gc) and 3-keto-3-deoxy-D-glycero-D-galactonononic acid (KDN) (Hopkins et al. 2013). Peter et al. 2024 have proposed that conformational coupling of the sialic acid TRAP transporter HiSiaQM with its substrate binding protein HiSiaP accounts for its energetic features. The SBP can adopt an open- or closed state depending on the presence of substrate. The two transmembrane domains of TRAP transporters form a monomeric elevator whose function is strictly dependent on the presence of a sodium ion gradient (Peter et al. 2024).  cryo-EM structure of the Haemophilus influenzae N-acetylneuraminate TRAP transporter (HiSiaQM) at 2.99 Å resolution (extending to 2.2 Å at the core), revealed new features (Currie et al. 2024). .

Accession Number:P44543
Protein Name:SiaT aka Sialic acid TRAP transporter permease protein
Length:616
Molecular Weight:67593.00
Species:Haemophilus influenzae [727]
Number of TMSs:15
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate sodium(1+), N-glycoloyl-beta-neuraminic acid, N-acetyl-beta-neuraminic acid

Cross database links:

RefSeq: NP_438316.1   
Entrez Gene ID: 951057   
Pfam: PF06808    PF04290   
BioCyc: HINF71421:HI_0147-MONOMER   
KEGG: hin:HI0147   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0008643 P:carbohydrate transport

References (2)

[1] “Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.”  Fleischmann R.D.et.al.   7542800
[2] “Sialic acid transport in Haemophilus influenzae is essential for lipopolysaccharide sialylation and serum resistance and is dependent on a novel tripartite ATP-independent periplasmic transporter.”  Severi E.et.al.   16262798

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FASTA formatted sequence
1:	MKYINKLEEW LGGALFIAIF GILIAQILSR QVFHSPLIWS EELAKLLFVY VGMLGISVAV 
61:	RKQEHVFIDF LTNLMPEKIR KFTNTFVQLL VFICIFLFIH FGIRTFNGAS FPIDALGGIS 
121:	EKWIFAALPV VAILMMFRFI QAQTLNFKTG KSYLPATFFI ISAVILFAIL FFAPDWFKVL 
181:	RISNYIKLGS SSVYVALLVW LIIMFIGVPV GWSLFIATLL YFSMTRWNVV NAATEKLVYS 
241:	LDSFPLLAVP FYILTGILMN TGGITERIFN FAKALLGHYT GGMGHVNIGA SLLFSGMSGS 
301:	ALADAGGLGQ LEIKAMRDAG YDDDICGGIT AASCIIGPLV PPSIAMIIYG VIANESIAKL 
361:	FIAGFIPGVL ITLALMAMNY RIAKKRGYPR TPKATREQLC SSFKQSFWAI LTPLLIIGGI 
421:	FSGLFSPTES AIVAAAYSVI IGKFVYKELT LKSLFNSCIE AMAITGVVAL MIMTVTFFGD 
481:	MIAREQVAMR VADVFVAVAD SPLTVLIMIN ALLLFLGMFI DALALQFLVL PMLIPIAMQF 
541:	NIDLIFFGVM TTLNMMVGIL TPPMGMALFV VARVGNMSVS TVTKGVLPFL IPVFVTLVLI 
601:	TIFPQIITFV PNLLIP