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2.A.57.1.5
The brain plasma membrane monoamine transporter, PMAT, Slc29A4 or ENT4, a polyspecific orgnaocation transporter. (transports serotonin [Km=110 μM), dopamine (Km=330 μM), metformin (Km=1.3 mM) and the neurotoxin, 1-methyl-4-phenylpyridinium (Km=33 μM)) (Nucleosides and nucleobases are not transported; transport is sensitive to the membrane potential, but is Na+ and Cl- independent.) (Engel et al., 2004).  Also expressed in kidney apical membranes where it transports MPP+ by a ΔΨ-dependent process (Xia et al., 2007). TMSs 1 - 6 bear the substrate recognition site, and Glu206 in TMS5 determines the catioin specificiity. An E206Q mutant lost cation selectivity and transported uridine (Zhou et al. 2007). Residues Ile89 and thr220 influence its organic cation transport activity and sensitivity to inhibition by dilazep (Ho et al., 2012).  May play a role in insulin secretion in β-cells (Kobayashi et al. 2016). Overexpression of SLC29A4 may provide a new option to stratify patients with high-risk neuroblastoma for treatment with 131I-mIBG (Evers 2023).

Accession Number:Q7RTT9
Protein Name:Equilibrative nucleoside transporter 4 aka Brain transport protein PMAT
Length:530
Molecular Weight:58059.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate serotonin, dopamine, N-methyl-4-phenylpyridinium, metformin

Cross database links:

RefSeq: NP_001035751.1    NP_694979.2   
Entrez Gene ID: 222962   
Pfam: PF01733   
OMIM: 609149  gene
KEGG: hsa:222962    hsa:222962   

Gene Ontology

GO:0016324 C:apical plasma membrane
GO:0016021 C:integral to membrane
GO:0005337 F:nucleoside transmembrane transporter activity
GO:0006810 P:transport
GO:0005886 C:plasma membrane
GO:0015858 P:nucleoside transport
GO:0055085 P:transmembrane transport

References (16)

[1] “Identification and characterization of a novel monoamine transporter in the human brain.”  Engel K.et.al.   15448143
[2] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[3] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[4] “Molecular evolution of the equilibrative nucleoside transporter family: identification of novel family members in prokaryotes and eukaryotes.”  Acimovic Y.et.al.   12446811
[5] “Distribution and functional characterization of equilibrative nucleoside transporter-4, a novel cardiac adenosine transporter activated at acidic pH.”  Barnes K.et.al.   16873718
[6] “Membrane localization and pH-dependent transport of a newly cloned organic cation transporter (PMAT) in kidney cells.”  Xia L.et.al.   17018840
[7] “Molecular determinants of substrate selectivity of a novel organic cation transporter (PMAT) in the SLC29 family.”  Zhou M.et.al.   17121826
[8] “The organic cation transporters (OCT1, OCT2, EMT) and the plasma membrane monoamine transporter (PMAT) show differential distribution and cyclic expression pattern in human endometrium and early pregnancy decidua.”  Bottalico B.et.al.   17393420
[9] “Identification and characterization of a novel monoamine transporter in the human brain.”  Engel K.et.al.   15448143
[10] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[11] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[12] “Molecular evolution of the equilibrative nucleoside transporter family: identification of novel family members in prokaryotes and eukaryotes.”  Acimovic Y.et.al.   12446811
[13] “Distribution and functional characterization of equilibrative nucleoside transporter-4, a novel cardiac adenosine transporter activated at acidic pH.”  Barnes K.et.al.   16873718
[14] “Membrane localization and pH-dependent transport of a newly cloned organic cation transporter (PMAT) in kidney cells.”  Xia L.et.al.   17018840
[15] “Molecular determinants of substrate selectivity of a novel organic cation transporter (PMAT) in the SLC29 family.”  Zhou M.et.al.   17121826
[16] “The organic cation transporters (OCT1, OCT2, EMT) and the plasma membrane monoamine transporter (PMAT) show differential distribution and cyclic expression pattern in human endometrium and early pregnancy decidua.”  Bottalico B.et.al.   17393420

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MGSVGSQRLE EPSVAGTPDP GVVMSFTFDS HQLEEAAEAA QGQGLRARGV PAFTDTTLDE 
61:	PVPDDRYHAI YFAMLLAGVG FLLPYNSFIT DVDYLHHKYP GTSIVFDMSL TYILVALAAV 
121:	LLNNVLVERL TLHTRITAGY LLALGPLLFI SICDVWLQLF SRDQAYAINL AAVGTVAFGC 
181:	TVQQSSFYGY TGMLPKRYTQ GVMTGESTAG VMISLSRILT KLLLPDERAS TLIFFLVSVA 
241:	LELLCFLLHL LVRRSRFVLF YTTRPRDSHR GRPGLGRGYG YRVHHDVVAG DVHFEHPAPA 
301:	LAPNESPKDS PAHEVTGSGG AYMRFDVPRP RVQRSWPTFR ALLLHRYVVA RVIWADMLSI 
361:	AVTYFITLCL FPGLESEIRH CILGEWLPIL IMAVFNLSDF VGKILAALPV DWRGTHLLAC 
421:	SCLRVVFIPL FILCVYPSGM PALRHPAWPC IFSLLMGISN GYFGSVPMIL AAGKVSPKQR 
481:	ELAGNTMTVS YMSGLTLGSA VAYCTYSLTR DAHGSCLHAS TANGSILAGL