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2.A.6.2.13
Multidrug/dye/detergent resistance protein, YhiU/YhiV or MdtE/MdtF (Nishino and Yamaguchi 2001) MdtE (YhiU) is listed under TC# 8.A.1.6.3.  The system exports erythromycin, doxorubicin, crystal violet, ethidium, rhodamine 6G, TPP, benzalkonium, SDS, deoxycholate and growth inhibitory steroid hormones (estradiol and progesterone) (Elkins and Mullis, 2006).  Drug binding domain and channel conformational plasticity likely governs promiscuous substrate specificity, analogous to its closely related, constitutively expressed counterpart, AcrB (Lawrence et al. 2025). Distinct transmembrane state transitions within MdtF create a more engaged proton relay network, altered drug transport allostery and an acid-responsive increase in efflux efficiency. Physiologically, this provides means of xenobiotic and metabolite disposal within remodelled cell membranes that presage encounters with acid stresses, as endured in the gastrointestinal tract (Lawrence et al. 2025).

Accession Number:P37637
Protein Name:YhiV aka B3514
Length:1037
Molecular Weight:111517.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate rhodamine 6G, crystal violet, doxorubicin, 17beta-estradiol, progesterone, erythromycin A, benzalkonium chloride, sodium dodecyl sulfate, deoxycholate, thiamine(1+) diphosphate, ethidium

Cross database links:

RefSeq: AP_004279.1    NP_417971.1   
Entrez Gene ID: 948030   
Pfam: PF00873   
BioCyc: EcoCyc:YHIV-MONOMER    ECOL168927:B3514-MONOMER   
KEGG: ecj:JW3482    eco:b3514   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity
GO:0046677 P:response to antibiotic
GO:0006810 P:transport

References (10)

[1] “Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes.”  Sofia H.J.et.al.   8041620
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Analysis of a complete library of putative drug transporter genes in Escherichia coli.”  Nishino K.et.al.   11566977
[5] “EvgA of the two-component signal transduction system modulates production of the yhiUV multidrug transporter in Escherichia coli.”  Nishino K.et.al.   11914367
[6] “Escherichia coli gene expression responsive to levels of the response regulator EvgA.”  Masuda N.et.al.   12399493
[7] “Transcriptional regulation of drug efflux genes by EvgAS, a two-component system in Escherichia coli.”  Eguchi Y.et.al.   14523115
[8] “Regulatory network of acid resistance genes in Escherichia coli.”  Masuda N.et.al.   12694615
[9] “Protein complexes of the Escherichia coli cell envelope.”  Stenberg F.et.al.   16079137
[10] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MANYFIDRPV FAWVLAIIMM LAGGLAIMNL PVAQYPQIAP PTITVSATYP GADAQTVEDS 
61:	VTQVIEQNMN GLDGLMYMSS TSDAAGNASI TLTFETGTSP DIAQVQVQNK LQLAMPSLPE 
121:	AVQQQGISVD KSSSNILMVA AFISDNGSLN QYDIADYVAS NIKDPLSRTA GVGSVQLFGS 
181:	EYAMRIWLDP QKLNKYNLVP SDVISQIKVQ NNQISGGQLG GMPQAADQQL NASIIVQTRL 
241:	QTPEEFGKIL LKVQQDGSQV LLRDVARVEL GAEDYSTVAR YNGKPAAGIA IKLAAGANAL 
301:	DTSRAVKEEL NRLSAYFPAS LKTVYPYDTT PFIEISIQEV FKTLVEAIIL VFLVMYLFLQ 
361:	NFRATIIPTI AVPVVILGTF AILSAVGFTI NTLTMFGMVL AIGLLVDDAI VVVENVERVI 
421:	AEDKLPPKEA THKSMGQIQR ALVGIAVVLS AVFMPMAFMS GATGEIYRQF SITLISSMLL 
481:	SVFVAMSLTP ALCATILKAA PEGGHKPNAL FARFNTLFEK STQHYTDSTR SLLRCTGRYM 
541:	VVYLLICAGM AVLFLRTPTS FLPEEDQGVF MTTAQLPSGA TMVNTTKVLQ QVTDYYLTKE 
601:	KDNVQSVFTV GGFGFSGQGQ NNGLAFISLK PWSERVGEEN SVTAIIQRAM IALSSINKAV 
661:	VFPFNLPAVA ELGTASGFDM ELLDNGNLGH EKLTQARNEL LSLAAQSPNQ VTGVRPNGLE 
721:	DTPMFKVNVN AAKAEAMGVA LSDINQTIST AFGSSYVNDF LNQGRVKKVY VQAGTPFRML 
781:	PDNINQWYVR NASGTMAPLS AYSSTEWTYG SPRLERYNGI PSMEILGEAA AGKSTGDAMK 
841:	FMADLVAKLP AGVGYSWTGL SYQEALSSNQ APALYAISLV VVFLALAALY ESWSIPFSVM 
901:	LVVPLGVVGA LLATDLRGLS NDVYFQVGLL TTIGLSAKNA ILIVEFAVEM MQKEGKTPIE 
961:	AIIEAARMRL RPILMTSLAF ILGVLPLVIS HGAGSGAQNA VGTGVMGGMF AATVLAIYFV 
1021:	PVFFVVVEHL FARFKKA