2.A.62.1.5
Sodium/proton antiporter, NhaD1, of 488 aas and 14 TMSs. Two NhaD homologues that catalyze Na+/H+ antiport were identified from this halotolerant and alkaliphilic strain isolated from sodium enriched black liquor. They exhibit 72% identity and have similar binding affinities for Na+ and Li+, while having different pH profiles. Ha-NhaD1 was active at pH 6.0 and most active at pH 8.0-8.5, whereas Ha-NhaD2 lacked activity at pH 6.0 but exhibited maximal activity at pH 9.5 or higher. Residues involved in transport were identified (Cui et al. 2016).
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| Accession Number: | ANA11559.1 |
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| Protein Name: | ANA11559.1 NhaD type Na+/H+ antiporter [Halomonas sp. Y2] |
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| Length: | 489 |
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| Molecular Weight: | |
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| Species: | Halomonas sp. Y2 [408656] |
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| Number of TMSs: | 14 |
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| Substrate |
lithium(1+), sodium(1+) |
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1: MKPPNALAAR HSSRRAAWLL TALCLTLLPS FAFAAAGPLD LTSSLTGIAA VTIFILAYAL
61: VMSEEIIHMR KSKPVLVAAG LIWAMVAWVY VQAGMPHEAE EAFKETLLEY AELLLFLLVA
121: MTYINAMEER RVFDTLRAWL VRKGFSYRTL FWITGCLAFA ISSIANNMTT AMLMCAVVLK
181: VAEGDNKFIS LCCVNVVVAS NAGGAFSPFG DITTLMVWQA GQVPFEGFFA LLVPAIVNFM
241: VPALIMNFFL VNRQPAALTE DVWMKRGAKR IILLFLVTVA ISVLCHSILY LPPAMGMMFG
301: LGLLQFFGYY LRRSLPRSLE RKRERYSRRG DWQKLEQLGS VVPFDIFSRI ARSEWDTLLF
361: FYGVVMCVGG LGFMGYLSLL SETLYGGWNP VWANVVLGLI SAVVDNIPVM FAVLSMAPDM
421: SEGNWLLITL TAGVGGSLLS MGSAAGVALM GQARGIYTFA VHLRWVPAIL LGYVASIAAH
481: LMINASMF