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2.A.7.17.2
General amino acid exporter (probably including aromatic amino acids as well as thr, met lys, glu and others), YddG.  Its topology with 10 TMSs and both the N- and C-termini inside has been established (Airich et al. 2010). This system has been used for the export of tryptophan for commercial purposes (Wang et al. 2013).  The 3-d structures (PD# 5I20) of a homologue (TC# 2.A.7.3.66) has been determined at 2.4 Å resolution, showing the outward facing conformation of a basket shaped structure with a central substrate binding cavity (Tsuchiya et al. 2016). Mutants of the yddG gene can export phenylalanine (Radi et al. 2022).

Accession Number:P46136
Protein Name:Aromatic amino acid exporter YddG
Length:293
Molecular Weight:31539.00
Species:Escherichia coli (strain K12) [83333]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate amino acid, lysine, methionine, threonine, phenylalanine, histidine, tryptophan, tyrosine, glutamate(2-)

Cross database links:

Entrez Gene ID: 945942   
Pfam: PF00892   
KEGG: eco:b1473   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015192 F:L-phenylalanine transmembrane transporter activity
GO:0015196 F:L-tryptophan transmembrane transporter activity
GO:0005302 F:L-tyrosine transmembrane transporter activity

References (8)

[1] “A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.”  Aiba H.et.al.   9097039
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Nitrate-inducible formate dehydrogenase in Escherichia coli K-12. I. Nucleotide sequence of the fdnGHI operon and evidence that opal (UGA) encodes selenocysteine.”  Berg B.L.et.al.   1834669
[5] “Detection of new genes in a bacterial genome using Markov models for three gene classes.”  Borodovsky M.et.al.   7567469
[6] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
[7] “YddG from Escherichia coli promotes export of aromatic amino acids.”  Doroshenko V.et.al.   17784858
[8] “Membrane topology analysis of the Escherichia coli aromatic amino acid efflux protein YddG.”  Airich L.G.et.al.   21042032

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MTRQKATLIG LIAIVLWSTM VGLIRGVSEG LGPVGGAAAI YSLSGLLLIF TVGFPRIRQI 
61:	PKGYLLAGSL LFVSYEICLA LSLGYAATHH QAIEVGMVNY LWPSLTILFA ILFNGQKTNW 
121:	LIVPGLLLAL VGVCWVLGGD NGLHYDEIIN NITTSPLSYF LAFIGAFIWA AYCTVTNKYA 
181:	RGFNGITVFV LLTGASLWVY YFLTPQPEMI FSTPVMIKLI SAAFTLGFAY AAWNVGILHG 
241:	NVTIMAVGSY FTPVLSSALA AVLLSAPLSF SFWQGALMVC GGSLLCWLAT RRG