2.A.8 The Gluconate:H
Protein members of the GntP family include known gluconate permeases of E. coli and Bacillus species (Peekhaus et al., 1997; Reizer et al., 1991). Four of the seven E. coli paralogues have been found to possess active gluconate uptake activity, and one of them (GntW) can accommodate both L-idonate and D-gluconate although L-idonate is the physiological substrate (Bausch et al., 1998). Another (GntP) transports D-gluconate with high affinity but is specifically induced by and transports D-fructuronate (Bates Utz et al., 2004). GntT of E. coli is the physiological gluconate permease (Porco et al., 1997). These proteins are of about 450 residues and possess 12 or 14 putative transmembrane α-helical spanners. The GntP family is in the IT superfamily (Prakash et al., 2003).
The generalized transport reaction catalyzed by proteins of the GntP family is:
Carbohydrate acid (out) + nH
This family belongs to the: IT Superfamily.
References associated with 2.A.8 family:
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Peekhaus, N. and T. Conway. (1998). Positive and negative transcriptional regulation of the Escherichia coli gluconate regulon gene gntT by GntR and the cyclic AMP (cAMP)-cAMP receptor protein complex. J. Bacteriol. 180: 1777-1785. 9537375
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Porco, A., N. Peekhaus, C. Bausch, S. Tong, T. Isturiz, and T. Conway. (1997). Molecular genetic characterization of the Escherichia coli gntT gene of GntI, the main system for gluconate metabolism. J. Bacteriol. 179: 1584-1590. 9045817
Prakash, S., G. Cooper, S. Singhi, and M.H. Saier, Jr. (2003). The ion transporter superfamily. Biochim. Biophys. Acta 1618: 79-92. 14643936
Reizer, A., J. Deutscher, M.H. Saier, Jr., and J. Reizer. (1991). Analysis of the gluconate (gnt) operon of Bacillus subtilis. Mol. Microbiol. 5: 1081-1089. 1659648
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Rodionov, D.A., P.S. Novichkov, E.D. Stavrovskaya, I.A. Rodionova, X. Li, M.D. Kazanov, D.A. Ravcheev, A.V. Gerasimova, A.E. Kazakov, G.Y. Kovaleva, E.A. Permina, O.N. Laikova, R. Overbeek, M.F. Romine, J.K. Fredrickson, A.P. Arkin, I. Dubchak, A.L. Osterman, and M.S. Gelfand. (2011). Comparative genomic reconstruction of transcriptional networks controlling central metabolism in the Shewanella genus. BMC Genomics 12Suppl1: S3. 21810205
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Tong, S., A. Porco, T. Isturiz, and T. Conway. (1996). Cloning and molecular genetic characterization of the Escherichia coli gntR, gntK, and gntU genes of GntI, the main system for gluconate metabolism. J. Bacteriol. 178: 3260-3269. 8655507
Yang, M., T. Mu, W. Zhong, A.M. Olajuyin, and J. Xing. (2017). Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes. Lett Appl Microbiol 65: 165-172. 28561260