2.A.85.2.7
The vacuolar malate "channel", ALMT9, of 598 aas and 6 - 8 TMSs. TMSs 1 - 6 occur together near the N-terminus; putatives TMS 7 is near the middle of the protein, and putative TMS 8 is near the C-terminus. Citrate is an open channel blocker. There are probably four subunits, and TMS5 contributes to pore formation (Zhang et al. 2013). It has higher selectivity for malate than for fumarate and exhibits weak chloride conductance (Kovermann et al. 2007). The apple ALMT9 requires a conserved C-terminal domain for malate transport, underlying fruit acidity (Li et al. 2020).
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| Accession Number: | Q9LS46 |
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| Protein Name: | Aluminum-activated malate transporter 9 |
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| Length: | 598 |
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| Molecular Weight: | 67046.00 |
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| Species: | Arabidopsis thaliana (Mouse-ear cress) [3702] |
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| Number of TMSs: | 6 |
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| Location1 / Topology2 / Orientation3: |
Vacuole membrane1 / Multi-pass membrane protein2 |
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| Substrate |
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1: MAAKQGSFRH GILEKRERLL SNNGFSDFRF TDIESNDLLE NENCGRRTRL CCCCSCGNLS
61: EKISGVYDDA KDVARKAWEM GVSDPRKIVF SAKIGLALTI VALLIFYQEP NPDLSRYSVW
121: AILTVVVVFE FTIGATLSKG FNRALGTLSA GGLALGMAEL STLFGDWEEI FCTLSIFCIG
181: FLATFMKLYP SMKAYEYGFR VFLLTYCYIL ISGFRTGQFI EVAISRFLLI ALGAGVSLGV
241: NMFIYPIWAG EDLHNLVVKN FMNVATSLEG CVNGYLRCLE YERIPSKILT YQASEDPVYK
301: GYRSAVESTS QEESLMSFAI WEPPHGPYKS FNYPWKNYVK LSGALKHCAF TVMALHGCIL
361: SEIQAPEERR QVFRQELQRV GVEGAKLLRE LGEKVKKMEK LGPVDLLFEV HLAAEELQHK
421: IDKKSYLLVN SECWEIGNRA TKESEPQELL SLEDSDPPEN HAPPIYAFKS LSEAVLEIPP
481: SWGEKNHREA LNHRPTFSKQ VSWPARLVLP PHLETTNGAS PLVETTKTYE SASALSLATF
541: ASLLIEFVAR LQNVVDAFKE LSQKANFKEP EIVTTGTDVE FSGERVGLGQ KIRRCFGM