3.A.1.111.7
Nukacin ISK-1 bacteriocin exporter, NukT of 694 aas and 6 TMSs. The protease domain is N-terminal, the membrane domain is central, and the ATPase domain in C-terminal. NukT and its peptidase-inactive mutant have been expressed, purified, and reconstituted into liposomes for analysis of their peptidase and ATPase activities. The ATPase activity of the NBD (C) region is required for the cysteine-type peptidase activity, and leader peptide cleavage enhances the ATPase activity (Zheng et al. 2017).
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| Accession Number: | Q75V16 |
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| Protein Name: | NukT |
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| Length: | 694 |
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| Molecular Weight: | 80129.00 |
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| Species: | Staphylococcus warneri [1292] |
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| Number of TMSs: | 6 |
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| Substrate |
peptide, protein polypeptide chain, bacteriocin |
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1: LKIALQNSDQ DCLLACYSMI LSYFGKNVSI NSLYKREMIP PDGLSISYLK ELNIKYELNM
61: KVYRIKDKEK TFRVISKIKK PIIVHWDLNH FVIVKNVKKK HIEIVNPEIG KVKISKEMFL
121: EHFSNVLLMF DPKSDFTKDK EKIEFFDDLK SVLMNKNIIL FSFSVMLAQI VALIFSIVVR
181: DIINQKYTYL ISLSMLLMMI IIQIFSLVFK QKAQIKENKI YEKIISYNMF EGLFNKPLLY
241: FRNSTIGTLM EKINIKTTIR DNILLKILPS FLNFFSVTVL FIYLLTVSLL LSLLLLGMSI
301: IYLLISVFIY IKKNQLNIEY MQRTIQFSSL AQESLSQIDQ IKAQAQESST INNWKEKSWN
361: IVNSYNSILK IEGLSYTFNQ IFNYSSIILL MIFGIHLTKY GNITIADLIL FQSGMSIFSS
421: SVSQLQDITF EISKIKVSGE KINDLFLKNN TKRKVIEQKS NNALSLKNVT FGFDEKAPIL
481: KNITFQVKKG EKVAIVGDSG SGKSTMLNVL LGLYTCDGEV VYGYRDFRKI TGVVLQNMTL
541: SKQSIYNNLV DEGNDSNMQK LNQILYDVNI INLINSLPNK IYSSVFQNGK NLSGGQIQRL
601: LIAKSLFNKK LIFWDEAFSS LDNYNRINIY RNVLKNSQYS DKTIVLISHH LDVLSYVDKV
661: MFIENGNAFF GTHNELIKNN KNYRRFLDTA NTNN